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Polymerase: T4 L771F

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Polymerase	Reference	Property	Result	Context
T4 L771F	A single mutation in bacteriophage T4 DNA polymerase (A737V, tsL141) decreases its processivity as a polymerase and increases its processivity as a 3'-->5' exonuclease.	Cloned or native	Cloned in E. coli
T4 L771F	A single mutation in bacteriophage T4 DNA polymerase (A737V, tsL141) decreases its processivity as a polymerase and increases its processivity as a 3'-->5' exonuclease.	Full length or truncated	Full length
T4 L771F	A single mutation in bacteriophage T4 DNA polymerase (A737V, tsL141) decreases its processivity as a polymerase and increases its processivity as a 3'-->5' exonuclease.	3-5' Exonuclease (proofreading)	Yes
T4 L771F	A single mutation in bacteriophage T4 DNA polymerase (A737V, tsL141) decreases its processivity as a polymerase and increases its processivity as a 3'-->5' exonuclease.	Specific Activity	2343 Other
T4 L771F	Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities.	Nucleotide Substitution Rate	0.3 Mutation frequency (relative to WT)	Technique: T4 rII 199oc reversion assay