A single mutation in bacteriophage T4 DNA polymerase (A737V, tsL141) decreases its processivity as a polymerase and increases its processivity as a 3'-->5' exonuclease.

Abstract:

The bacteriophage T4 DNA polymerase mutant A737V (tsL141 and tsCB120) ...

Polymerases:

Topics:

Mutational Analysis, Historical Protein Properties (MW, pI, ...), Nucleotide Incorporation, Exonuclease Activity, Source / Purification

One line summary:

T4 A737V is found to arrest much more frequently than its wild type variant during DNA synthesis; exonuclease activity is indistinguishable from its wild type on single-stranded DNA, while exonuclease activity on duplex DNA is more frequent.

Note:

Need to add pol: T4 A737V/L771F (double mutant)

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
T4 A single mutation in bacteriophage T4 DNA polymerase (A737V, tsL141) decreases its processivity as a polymerase and increases its processivity as a 3'-->5' exonuclease. 3-5' Exonuclease (proofreading) Yes
T4 A single mutation in bacteriophage T4 DNA polymerase (A737V, tsL141) decreases its processivity as a polymerase and increases its processivity as a 3'-->5' exonuclease. Cloned or native Cloned in E. coli
T4 A single mutation in bacteriophage T4 DNA polymerase (A737V, tsL141) decreases its processivity as a polymerase and increases its processivity as a 3'-->5' exonuclease. Full length or truncated Full length
T4 A single mutation in bacteriophage T4 DNA polymerase (A737V, tsL141) decreases its processivity as a polymerase and increases its processivity as a 3'-->5' exonuclease. Specific Activity 3039 units/mg
T4 A737V A single mutation in bacteriophage T4 DNA polymerase (A737V, tsL141) decreases its processivity as a polymerase and increases its processivity as a 3'-->5' exonuclease. 3-5' Exonuclease (proofreading) Yes
T4 A737V A single mutation in bacteriophage T4 DNA polymerase (A737V, tsL141) decreases its processivity as a polymerase and increases its processivity as a 3'-->5' exonuclease. Cloned or native Cloned in E. coli
T4 A737V A single mutation in bacteriophage T4 DNA polymerase (A737V, tsL141) decreases its processivity as a polymerase and increases its processivity as a 3'-->5' exonuclease. Full length or truncated Full length
T4 A737V A single mutation in bacteriophage T4 DNA polymerase (A737V, tsL141) decreases its processivity as a polymerase and increases its processivity as a 3'-->5' exonuclease. Processivity 18bp
T4 A737V A single mutation in bacteriophage T4 DNA polymerase (A737V, tsL141) decreases its processivity as a polymerase and increases its processivity as a 3'-->5' exonuclease. Specific Activity 741 units/mg
T4 L771F A single mutation in bacteriophage T4 DNA polymerase (A737V, tsL141) decreases its processivity as a polymerase and increases its processivity as a 3'-->5' exonuclease. 3-5' Exonuclease (proofreading) Yes
T4 L771F A single mutation in bacteriophage T4 DNA polymerase (A737V, tsL141) decreases its processivity as a polymerase and increases its processivity as a 3'-->5' exonuclease. Cloned or native Cloned in E. coli
T4 L771F A single mutation in bacteriophage T4 DNA polymerase (A737V, tsL141) decreases its processivity as a polymerase and increases its processivity as a 3'-->5' exonuclease. Full length or truncated Full length
T4 L771F A single mutation in bacteriophage T4 DNA polymerase (A737V, tsL141) decreases its processivity as a polymerase and increases its processivity as a 3'-->5' exonuclease. Specific Activity 2343 Other

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