Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities.

Stocki SA, Nonay RL, Reha-Krantz LJ
Journal of molecular biology (1995), Volume 254, Page 15
PubMed entry Publisher's site

Abstract:

Many DNA polymerases are multifunctional with the ability to replicate ...

Polymerases:

T4 W213S,T4 I417V,T4 A737V,T4 A777V,T4 I50L,T4 64[KD]uplication,T4 N84K,T4 257[KEIYS]uplication,T4 271[L]uplication,T4 V355A,T4 Q380K,T4 E395K,T4 L412M,T4 M725I,T4 M725V,T4 E743K,T4 S756P,T4 L771F,T4 L771H,T4 [L771+D],T4 [L771+V],T4 G255S,T4 G255A,T4

Topics:

Mutational Analysis, Kinetic Parameters, Structure and Structure/Function, Fidelity, Exonuclease Activity

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
T4 I50L Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. Nucleotide Substitution Rate 100 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 64[KD]uplication Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. Nucleotide Substitution Rate 50 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 N84K Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. Nucleotide Substitution Rate 100 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 G255S Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. Nucleotide Substitution Rate 58 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 271[L]uplication Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. Nucleotide Substitution Rate 74 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 V355A Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. Nucleotide Substitution Rate 5 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 Q380K Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. Nucleotide Substitution Rate 5 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 E395K Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. Nucleotide Substitution Rate 14 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 L412M Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. Nucleotide Substitution Rate 9 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 E743K Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. Nucleotide Substitution Rate 11 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 S756P Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. Nucleotide Substitution Rate 13 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 L771F Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. Nucleotide Substitution Rate 0.3 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 L771H Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. Nucleotide Substitution Rate 8 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 [L771+D] Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. Nucleotide Substitution Rate 29 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 [L771+V] Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. Nucleotide Substitution Rate 22 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
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