The initial step of in vitro synthesis of deoxyribonucleic acid by the T4 deoxyribonucleic acid polymerase.

Englund PT
The Journal of biological chemistry (1971), Volume 246, Page 5684
PubMed entry

Polymerases:

T4,T7

Topics:

Historical Protein Properties (MW, pI, ...), Nucleotide Incorporation, Exonuclease Activity

One line summary:

Demonstration that 3' exonuclease activity of the T4 DNA polymerase trims unpaired 3'-ends of hairpin structures to create paired duplex ends for DNA synthesis.

Status:

new topics/pols set partial results complete validated

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