The initial step of in vitro synthesis of deoxyribonucleic acid by the T4 deoxyribonucleic acid polymerase.

Polymerases:

T4,T7

Topics:

Historical Protein Properties (MW, pI, ...), Nucleotide Incorporation, Exonuclease Activity

One line summary:

Demonstration that 3' exonuclease activity of the T4 DNA polymerase trims unpaired 3'-ends of hairpin structures to create paired duplex ends for DNA synthesis.

Status:

new topics/pols set partial results complete validated

Results:

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