Construction of biologically functional bacterial plasmids in vitro.

Cohen SN, Chang AC, Boyer HW, Helling RB
Proceedings of the National Academy of Sciences of the United States of America (1973), Volume 70, Page 3240
PubMed entry Full article text

Abstract:

The construction of new plasmid DNA species by in vitro joining of ...
The construction of new plasmid DNA species by in vitro joining of restriction endonuclease-generated fragments of separate plasmids is described. Newly constructed plasmids that are inserted into Escherichia coli by transformation are shown to be biologically functional replicons that possess genetic properties and nucleotide base sequences from both of the parent DNA molecules. Functional plasmids can be obtained by reassociation of endonuclease-generated fragments of larger replicons, as well as by joining of plasmid DNA molecules of entirely different origins.

Polymerases:

Topics:

Historical Protein Properties (MW, pI, ...), Other Enzymatic Activities

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