RNA-dependent DNA polymerase activity of RNA tumor virus. VI. Processive mode of action of avian myeloblastosis virus polymerase.

Abstract:

Purified avian myeloblastosis virus (AMV) polymerase consisting of alpha,beta subunits has been shown to act processively in catalyzing DNA synthesis primed with 34S AMV RNA oligo(dT), poly(A)-poly(dT), and poly(I)-poly(dC). DNA transcripts prepared with 34S AMV RNA-oligo(dT)14 and AMV polymerase (alphabeta) have been shown to have a molecular weight of 1.05 X 10(6), or approximately one-third the size of the 34S RNA genome. Polymerase subunit alpha acts nonprocessively with the above templates.

Polymerases:

AMV

Topics:

Reverse Transcriptase, Historical Protein Properties (MW, pI, ...), Source / Purification, RNase H Activity, Kinetic Parameters

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
AMV Leis JP1976 Reverse Transcriptase Activity Yes
AMV Leis JP1976 Molecular Weight 1.73E+05 Dalton
AMV Leis JP1976 Cloned or native Native organism
AMV Leis JP1976 Tagged No
AMV Leis JP1976 Full length or truncated Full length
AMV Leis JP1976 RNase H Yes
AMV Leis JP1976 KM 10uM Reaction: Nucleotide incorporation; Substrate: dNTPs

Entry validated by:

Log in to edit reference All References

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.