Techniques used to study the DNA polymerase reaction pathway.

Joyce CM
Biochimica et biophysica acta (2010), Volume 1804, Page 1032
PubMed entry Full article text Publisher's site

Abstract:

A minimal reaction pathway for DNA polymerases was established over ...
A minimal reaction pathway for DNA polymerases was established over 20years ago using chemical-quench methods. Since that time there has been considerable interest in noncovalent steps in the reaction pathway, conformational changes involving the polymerase or its DNA substrate that may play a role in substrate specificity. Fluorescence-based assays have been devised in order to study these conformational transitions and the results obtained have added new detail to the reaction pathway.

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