Removal of contaminating TEM-la beta-lactamase gene from commercial Taq DNA polymerase.

Song JS, Lee JH, Lee JH, Jeong BC, Lee WK, Lee SH
J Microbiol (2006), Volume 44, Page 126
PubMed entry

Abstract:

This study confirms that Taq DNA polymerase could be contaminated with ...
This study confirms that Taq DNA polymerase could be contaminated with the blaTEM-1(a) gene. It also proposes two different methods that could be used to overcome DNA contamination: (i) DNase I treatment prior to PCR amplification; and (ii) the use of a highly purified Taq DNA polymerase which was devoid of detectable contamination.

Polymerases:

Taq pol I

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