Removal of contaminating TEM-la beta-lactamase gene from commercial Taq DNA polymerase.

J Microbiol (2006), Volume 44, Page 126


This study confirms that Taq DNA polymerase could be contaminated with the blaTEM-1(a) gene. It also proposes two different methods that could be used to overcome DNA contamination: (i) DNase I treatment prior to PCR amplification; and (ii) the use of a highly purified Taq DNA polymerase which was devoid of detectable contamination.




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