Usefulness of repeated GenomiPhi, a phi29 DNA polymerase-based rolling circle amplification kit, for generation of large amounts of plasmid DNA.

Sato M, Ohtsuka M, Ohmi Y
Biomol Eng (2005), Volume 22, Page 129
PubMed entry Publisher's site

Abstract:

The GenomiPhi DNA Amplification Kit employs rolling circle ...
The GenomiPhi DNA Amplification Kit employs rolling circle amplification (RCA) using phi29 polymerase, dNTPs, and random hexamers. We demonstrated that repeated RCA (at least three times) is useful for high-fidelity amplification of large amounts of plasmid DNA.

Polymerases:

Phi29

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