Phi29

From Bacteriophage φ29 (aliases:φ29)
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Welcome to the Polymerase Page

This page presents all the information in Polbase for Phi29.

Mutants:

At the top of the page you'll find a map of all positions of known mutants. Text links to mutants and digestion products are listed in the Mutants section.

Structures/Sequence:

If available, a representative structure is displayed at the upper right. All known structures are listed in the Structures section.

External links:

Polbase polymerases are linked to UniProt and Genbank where possible. The UniProt search link directs you to a pre-constructed UniProt search containing relevant terms.

References:

All references that refer to this Polymerase are listed with their topics and paper's title in the References table.

Results:

All the results for this polymerase can be examined by following the "Results" link at the bottom of the page.

Help icons:

The show help symbol in the upper-right corner of the page links to this help text. The question mark icon is used everywhere to indicate that help is available.

Phi29 is a family B enzyme from Bacteriophage φ29.

Selected Properties for Phi29:

3'-5' exo activity 5'-3' exo activity Frameshift Rate Substitution Rate General Error Rate
Yes (8) no data no data no data 3.0e-06 errors/bp (1)
Year Authors Title Results by property
2013 Alicia Del Prado, José María Lázaro, Laurentino Villar, Margarita Salas, Miguel de Vega Dual Role of φ29 DNA Polymerase Lys52...
2012 Elizabeth A Manrao, Ian M Derrington, Andrew H Laszlo, Kyle W Langford, Matthew K Hopper, Nathaniel Gillgren, Mikhail Pavlenok, Michael Niederweis, Jens H Gundlach Reading DNA at single-nucleotide reso...
2012 José A Morin, Francisco J Cao, José María Lázaro, J Ricardo Arias-Gonzalez, José M Valpuesta, José L Carrascosa, Margarita Salas, Borja Ibarra Active DNA unwinding dynamics during ...
2012 Joseph M Dahl, Ai H Mai, Gerald M Cherf, Nahid N Jetha, Daniel R Garalde, Andre Marziali, Mark Akeson, Hongyun Wang, Kate R Lieberman Direct observation of translocation i...
2012 Kate R Lieberman, Joseph M Dahl, Ai H Mai, Mark Akeson, Hongyun Wang Dynamics of the translocation step me...
2012 Xu Hun, Fang Liu, Zhenhua Mei, Lifeng Ma, Zhouping Wang, Xiliang Luo Signal amplified strategy based on ta...
2011 Mario Mencía, Pablo Gella, Ana Camacho, Miguel de Vega, Margarita Salas Terminal protein-primed amplification...
2011 Noël P Burtt Whole-genome amplification using Φ29 ...
2010 Patricia Pérez-Arnaiz, José María Lázaro, Margarita Salas, Miguel de Vega phi29 DNA polymerase active site: rol...
2010 Miguel de Vega, José María Lázaro, Mario Mencía, Luis Blanco, Margarita Salas Improvement of phi29 DNA polymerase a...
2010 Kate R Lieberman, Gerald M Cherf, Michael J Doody, Felix Olasagasti, Yvette Kolodji, Mark Akeson Processive replication of single DNA ...
2009 Arunas Lagunavicius, Egle Merkiene, Zivile Kiveryte, Agne Savaneviciute, Vilma Zimbaite-Ruskuliene, Tomas Radzvilavicius, Arvydas Janulaitis Novel application of Phi29 DNA polyme...
2009 Irene Rodríguez, José María Lázaro, Margarita Salas, Miguel de Vega Involvement of the TPR2 subdomain mov...
2009 Patricia Pérez-Arnaiz, José María Lázaro, Margarita Salas, Miguel de Vega Functional importance of bacteriophag... 3-5' exo,5-3' exo,All properties
2008 Margarita Salas, Luis Blanco, José María Lázaro, Miguel de Vega The bacteriophage phi29 DNA polymerase.
2008 Yun Xu, Simon Gao, John F Bruno, Benjamin J Luft, John J Dunn Rapid detection and identification of...
2008 Kaisa Silander, Janna Saarela Whole genome amplification with Phi29...
2008 Arunas Lagunavicius, Zivile Kiveryte, Vilma Zimbaite-Ruskuliene, Tomas Radzvilavicius, Arvydas Janulaitis Duality of polynucleotide substrates ... RNase H,All properties
2008 Gemma Serrano-Heras, Alicia Bravo, Margarita Salas Phage phi29 protein p56 prevents vira...
2008 Linda J Reha-Krantz Recent patents of gene sequences rela...
2007 Patricia Pérez-Arnaiz, Elisa Longás, Laurentino Villar, José María Lázaro, Margarita Salas, Miguel de Vega Involvement of phage phi29 DNA polyme...
2007 Andrea J Berman, Satwik Kamtekar, Jessica L Goodman, José María Lázaro, Miguel de Vega, Luis Blanco, Margarita Salas, Thomas A Steitz Structures of phi29 DNA polymerase co...
2006 Satwik Kamtekar, Andrea J Berman, Jianhong Wang, José María Lázaro, Miguel de Vega, Luis Blanco, Margarita Salas, Thomas A Steitz The phi29 DNA polymerase:protein-prim...
2006 Patricia Pérez-Arnaiz, José María Lázaro, Margarita Salas, Miguel de Vega Involvement of phi29 DNA polymerase t...
2006 Elisa Longás, Miguel de Vega, José María Lázaro, Margarita Salas Functional characterization of highly... Cloned or native,Full length or truncated,3-5' exo,Vmax,All properties
2006 Thomas A Steitz Visualizing polynucleotide polymerase...
2005 Clyde A Hutchison, Hamilton O Smith, Cynthia Pfannkoch, J Craig Venter Cell-free cloning using phi29 DNA pol...
2005 Irene Rodríguez, José María Lázaro, Luis Blanco, Satwik Kamtekar, Andrea J Berman, Jianhong Wang, Thomas A Steitz, Margarita Salas, Miguel de Vega A specific subdomain in phi29 DNA pol... Full length or truncated,Processivity,3-5' exo,All properties
2005 Juan Luis Asensio, Armando Albert, Daniel Muñoz-Espín, Carlos Gonzalez, Juan Hermoso, Laurentino Villar, Jesús Jiménez-Barbero, Margarita Salas, Wilfried J J Meijer Structure of the functional domain of...
2005 Jianhong Wang, Satwik Kamtekar, Andrea J Berman, Thomas A Steitz Correction of X-ray intensities from ...
2005 Vijay Gadkar, Matthias C Rillig Application of Phi29 DNA polymerase m...
2005 Masahiro Sato, Masato Ohtsuka, Yuhsuke Ohmi Usefulness of repeated GenomiPhi, a p...
2005 Naoyuki Umetani, Michiel F G de Maat, Takuji Mori, Hiroya Takeuchi, Dave S B Hoon Synthesis of universal unmethylated c...
2005 Verónica Truniger, Ana Bonnin, José María Lázaro, Miguel de Vega, Margarita Salas Involvement of the "linker" region be... 3-5' exo,5-3' exo,All properties
2004 E Adachi, K Shimamura, S Wakamatsu, H Kodama Amplification of plant genomic DNA by...
2004 Irene Rodríguez, José María Lázaro, Margarita Salas, Miguel de Vega phi29 DNA polymerase-terminal protein...
2004 Víctor González-Huici, Martín Alcorlo, Margarita Salas, José M Hermoso Binding of phage Phi29 architectural ...
2004 C X Wang, E Zakharova, Jinming Li, Catherine M Joyce, Jianhong Wang, William H Konigsberg Pre-steady-state kinetics of RB69 DNA...
2004 Satwik Kamtekar, Andrea J Berman, Jianhong Wang, José María Lázaro, Miguel de Vega, Luis Blanco, Margarita Salas, Thomas A Steitz Insights into strand displacement and... Processivity,All properties
2004 Verónica Truniger, José María Lázaro, Margarita Salas Function of the C-terminus of phi29 D...
2004 Verónica Truniger, José María Lázaro, Margarita Salas Two positively charged residues of ph...
2004 Masahiro Sato, Masato Ohtsuka, Yuhsuke Ohmi Repeated GenomiPhi, phi29 DNA polymer...
2003 Verónica Truniger, José María Lázaro, Miguel de Vega, Luis Blanco, Margarita Salas phi 29 DNA polymerase residue Leu384,...
2003 Irene Rodríguez, José María Lázaro, Margarita Salas, Miguel de Vega phi29 DNA polymerase residue Phe128 o...
2002 Irene Gascón, José L Carrascosa, Laurentino Villar, José María Lázaro, Margarita Salas Importance of the N-terminal region o...
2002 Verónica Truniger, José María Lázaro, Francisco J Esteban, Luis Blanco, Margarita Salas A positively charged residue of phi29...
2002 Ralf Eisenbrandt, José María Lázaro, Margarita Salas, Miguel de Vega Phi29 DNA polymerase residues Tyr59, ... 3-5' exo,5-3' exo,All properties
2002 Verónica Truniger, José María Lázaro, Luis Blanco, Margarita Salas A highly conserved lysine residue in ...
2002 John R Nelson, Yuyang Christine Cai, Theresa L Giesler, Joseph W Farchaus, Shanmuuga T Sundaram, Maria Ortiz-Rivera, Lou P Hosta, Peter L Hewitt, J Anthony Mamone, C Palaniappan, Carl W Fuller TempliPhi, phi29 DNA polymerase based... Cloned or native,Tagged,Full length or truncated,Processivity,3-5' exo,Maximum Product Length,Vmax,Overall Error Rate,All properties
2001 F B Dean, John R Nelson, Theresa L Giesler, R S Lasken Rapid amplification of plasmid and ph...
2001 Wilfried J J Meijer, A Serna-Rico, Margarita Salas Characterization of the bacteriophage...
2001 Arjan B Brenkman, M R Heideman, Verónica Truniger, Margarita Salas, Peter C van der Vliet The (I/Y)XGG motif of adenovirus DNA ...
2001 Wilfried J J Meijer, J A Horcajadas, Margarita Salas Phi29 family of phages.
2000 V Gonzalez-Huici, José María Lázaro, Margarita Salas, José M Hermoso Specific recognition of parental term...
2000 Emmanuelle Dufour, Juan Méndez, José María Lázaro, Miguel de Vega, Luis Blanco, Margarita Salas An aspartic acid residue in TPR-1, a ...
2000 Miguel de Vega, José María Lázaro, Margarita Salas Phage phi 29 DNA polymerase residues ...
2000 Verónica Truniger, Luis Blanco, Margarita Salas Analysis of O29 DNA polymerase by par...
2000 A Serna-Rico, B Illana, Margarita Salas, Wilfried J J Meijer The putative coiled coil domain of th...
2000 Alicia Bravo, B Illana, Margarita Salas Compartmentalization of phage phi29 D...
2000 Irene Gascón, José María Lázaro, Margarita Salas Differential functional behavior of v...
1999 Ana Bonnin, José María Lázaro, Luis Blanco, Margarita Salas A single tyrosine prevents insertion ...
1999 Miguel de Vega, Luis Blanco, Margarita Salas Processive proofreading and the spati... Cloned or native,Full length or truncated,3-5' exo,5-3' exo,Vmax,All properties
1999 D Canceill, Enrique Viguera, S Dusko Ehrlich Replication slippage of different DNA...
1999 M Elías-Arnanz, Margarita Salas Resolution of head-on collisions betw...
1999 A M Abril, S Marco, José L Carrascosa, Margarita Salas, José M Hermoso Oligomeric structures of the phage ph...
1999 B Illana, José María Lázaro, C Gutiérrez, Wilfried J J Meijer, Luis Blanco, Margarita Salas Phage phi29 terminal protein residues...
1999 Verónica Truniger, Luis Blanco, Margarita Salas Role of the "YxGG/A" motif of Phi29 D...
1998 V Murthy, Wilfried J J Meijer, Luis Blanco, Margarita Salas DNA polymerase template switching at ...
1998 Miguel de Vega, José María Lázaro, Margarita Salas, Luis Blanco Mutational analysis of phi29 DNA poly...
1998 P Crucitti, José María Lázaro, V Benes, Margarita Salas Bacteriophage phi29 early protein p17...
1998 Miguel de Vega, Luis Blanco, Margarita Salas phi29 DNA polymerase residue Ser122, ...
1998 Verónica Truniger, José María Lázaro, Margarita Salas, Luis Blanco Phi 29 DNA polymerase requires the N-...
1998 B Illana, A Zaballos, Luis Blanco, Margarita Salas The RGD sequence in phage phi29 termi...
1998 J Saturno, José María Lázaro, Luis Blanco, Margarita Salas Role of the first aspartate residue o...
1997 J Mendez, Luis Blanco, Margarita Salas Protein-primed DNA replication: a tra...
1997 Alicia Bravo, Margarita Salas Initiation of bacteriophage phi29 DNA...
1997 J A Esteban, Luis Blanco, Laurentino Villar, Margarita Salas In vitro evolution of terminal protei...
1997 A M Abril, Margarita Salas, J M Andreu, José M Hermoso, G Rivas Phage phi29 protein p6 is in a monome...
1997 M Elías-Arnanz, Margarita Salas Bacteriophage phi29 DNA replication a...
1997 J Saturno, José María Lázaro, Francisco J Esteban, Luis Blanco, Margarita Salas o29 DNA polymerase residue Lys383, in...
1997 M S Soengas, C R Mateo, G Rivas, Margarita Salas, A U Acuña, C Gutiérrez Structural features of phi29 single-s...
1996 R Freire, M Serrano, Margarita Salas, José M Hermoso Activation of replication origins in ...
1996 B Illana, Luis Blanco, Margarita Salas Functional characterization of the ge...
1996 Luis Blanco, Margarita Salas Relating structure to function in phi...
1996 Miguel de Vega, J M Lazaro, Margarita Salas, Luis Blanco Primer-terminus stabilization at the ...
1995 José María Lázaro, Luis Blanco, Margarita Salas Purification of bacteriophage phi 29 ...
1995 Luis Blanco, Margarita Salas Mutational analysis of bacteriophage ...
1995 M A Blasco, Juan Méndez, José María Lázaro, Luis Blanco, Margarita Salas Primer terminus stabilization at the ...
1995 J Saturno, Luis Blanco, Margarita Salas, J A Esteban A novel kinetic analysis to calculate...
1994 J A Esteban, M S Soengas, Margarita Salas, Luis Blanco 3'-->5' exonuclease active site of ph...
1994 Luis Blanco, José María Lázaro, Miguel de Vega, Ana Bonnin, Margarita Salas Terminal protein-primed DNA amplifica... Maximum Product Length,All properties
1993 J A Esteban, Margarita Salas, Luis Blanco Fidelity of phi 29 DNA polymerase. Co...
1993 M A Blasco, José María Lázaro, Luis Blanco, Margarita Salas Phi 29 DNA polymerase active site. Th...
1992 DNA Replication
1992 M A Blasco, J A Esteban, Juan Méndez, Luis Blanco, Margarita Salas Structural and functional studies on ...
1992 J A Esteban, Antonio Bernad, Margarita Salas, Luis Blanco Metal activation of synthetic and deg...
1992 C Garmendia, Antonio Bernad, J A Esteban, Luis Blanco, Margarita Salas The bacteriophage phi 29 DNA polymera... Full length or truncated,3-5' exo,Molecular Weight,All properties
1992 M S Soengas, J A Esteban, José María Lázaro, Antonio Bernad, M A Blasco, Margarita Salas, Luis Blanco Site-directed mutagenesis at the Exo ...
1992 M A Blasco, José María Lázaro, Antonio Bernad, Luis Blanco, Margarita Salas Phi 29 DNA polymerase active site. Mu...
1992 Luis Blanco, Antonio Bernad, J A Esteban, Margarita Salas DNA-independent deoxynucleotidylation...
1991 M A Blasco, Antonio Bernad, Luis Blanco, Margarita Salas Characterization and mapping of the p...
1990 M A Blasco, Luis Blanco, E Parés, Margarita Salas, Antonio Bernad Structural and functional analysis of...
1990 Antonio Bernad, Luis Blanco, Margarita Salas Site-directed mutagenesis of the YCDT...
1990 Antonio Bernad, José María Lázaro, Margarita Salas, Luis Blanco The highly conserved amino acid seque...
1990 C Garmendia, José M Hermoso, Margarita Salas Functional domain for priming activit...
1989 Luis Blanco, Antonio Bernad, José María Lázaro, G Martín, C Garmendia, Margarita Salas Highly efficient DNA synthesis by the... Full length or truncated,Processivity,Molecular Weight,All properties
1989 A Zaballos, Margarita Salas Functional domains in the bacteriopha...
1989 A Zaballos, José María Lázaro, E Méndez, R P Mellado, Margarita Salas Effects of internal deletions on the ...
1988 A Zaballos, R P Mellado, Margarita Salas Initiation of phage phi 29 DNA replic...
1988 C Garmendia, Margarita Salas, José M Hermoso Site-directed mutagenesis in the DNA ...
1987 Luis Blanco, I Prieto, J Gutiérrez, Antonio Bernad, José María Lázaro, José M Hermoso, Margarita Salas Effect of NH4+ ions on phi 29 DNA-pro...
1986 A Zaballos, Margarita Salas, R P Mellado Initiation of phage phi 29 DNA replic...
1986 J Gutiérrez, J Vinós, I Prieto, E Méndez, José M Hermoso, Margarita Salas Signals in the phi 29 DNA-terminal pr...
1986 Luis Blanco, Margarita Salas Effect of aphidicolin and nucleotide ...
1986 P García, José M Hermoso, J A García, E García, R López, Margarita Salas Formation of a covalent complex betwe...
1985 Luis Blanco, Margarita Salas Characterization of a 3'----5' exonuc...
1985 José M Hermoso, E Méndez, F Soriano, Margarita Salas Location of the serine residue involv...
1985 Luis Blanco, Margarita Salas Replication of phage phi 29 DNA with ...
1984 Margarita Salas A new mechanism for the initiation of...
1984 I Prieto, José María Lázaro, J A García, José M Hermoso, Margarita Salas Purification in a functional form of ...
1984 Luis Blanco, Margarita Salas Characterization and purification of ... Cloned or native,Tagged,Full length or truncated,Molecular Weight,Other accessory protein(s),All properties
1984 M G Blanco, C Hardisson, J A Salas Resistance in inhibitors of RNA polym...
1983 R P Mellado, Margarita Salas Initiation of phage phi 29 DNA replic...
1982 M A Peñalva, Margarita Salas Initiation of phage phi 29 DNA replic... Cloned or native,Tagged,Other accessory protein(s),All properties
1981 S Krawiec, F Jiménez, J A García, N Villanueva, J Sogo, Margarita Salas The orderly, in vitro emergence of DN...
1981 José L Carrascosa, E Méndez, J Corral, V Rubio, G Ramírez, Margarita Salas, E Viñuela Structural organization of Bacillus s...
1980 J Kuznar, María L Salas, E Viñuela DNA-dependent RNA polymerase in Afric...
1979 J M Sogo, P Rodeño, T Koller, E Viñuela, Margarita Salas Comparison of the A-T rich regions an...
1979 J M Sogo, M R Inciarte, J Corral, E Viñuela, Margarita Salas RNA polymerase binding sites and tran...
1978 Margarita Salas, R P Mellado, E Viñuela Characterization of a protein covalen...
1977 Ana Camacho, F Jiménez, J De La Torre, José L Carrascosa, R P Mellado, C Vásquez, E Viñuela, Margarita Salas Assembly of Bacillus subtilis phage p...
1976 José L Carrascosa, Ana Camacho, F Moreno, F Jiménez, R P Mellado, E Viñuela, Margarita Salas Bacillus subtilis phage phi29. Charac...
1976 E Viñuela, Ana Camacho, F Jiménez, José L Carrascosa, G Ramírez, Margarita Salas Structure and assembly of phage phi29.
1976 M R Inciarte, E Viñuela, Margarita Salas Transcription in vitro of phi29 DNA a...
1976 M R Inciarte, José María Lázaro, Margarita Salas, E Vińuela Physical map of bacteriophage phi29 DNA.
1975 José L Carrascosa, F Jiménez, E Viñuela, Margarita Salas Synthesis in vitro of phi29-specific ...
1975 Ana Camacho, José L Carrascosa, E Vinuela, Margarita Salas Discrepancy in the mobility of a prot...
1974 F Jiménez, J Avila, E Viñuela, Margarita Salas Initiation of the transcription of ph...
1974 V Rubio, Margarita Salas, E Viñuela, P Usobiaga, J L Saiz, J F Llopis Biophysical properties of bacteriopha...
1973 José L Carrascosa, E Viñuela, Margarita Salas Proteins induced in Bacillus subtilis...
1972 G Ramírez, E Méndez, Margarita Salas, E Viñuela Head-neck connecting protein in phage...
1972 G Alvarez, E Salas, N Pérez, J E Celis Phi29 bacteriophage structural proteins.
1972 José M Hermoso, J Avila, F Jiménez, Margarita Salas RNA polymerase from Bacillus amyloliq...
1972 A Talavera, Margarita Salas, E Viñuela Temperature-sensitive mutants affecte...
1971 J Avila, José M Hermoso, E Vinuela, Margarita Salas Purification and properties of DNA-de...
1970 J Avila, José M Hermoso, E Viñuela, Margarita Salas Subunit composition of B. subtilis RN...
Protein sequence:

Using Polbase tables:

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