Development of a new DNA sequencing method: 3'-ester cleavage catalyzed by Taq DNA polymerase.

Rasolonjatovo I, Sarfati SR
Nucleosides Nucleotides (1999), Volume 18, Page 1021
PubMed entry

Abstract:

A new 3'-esterified dTTP is incorporated into DNA by Taq DNA ...
A new 3'-esterified dTTP is incorporated into DNA by Taq DNA polymerase but does not act as a chain terminator. The esterase activity of the polymerase seems to be template dependent and occurs only if the next correct nucleotide is present.

Polymerases:

Taq pol I

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