Direct radioactive labeling of unpurified PCR products using Klenow fragment.

McDaniel TK, Huang Y, Yin J, Needleman SW, Meltzer SJ
BioTechniques (1991), Volume 11, Page 164
PubMed entry

Abstract:

We describe a method for rapid radioactive labeling of PCR product. ...
We describe a method for rapid radioactive labeling of PCR product. The method, employing the Klenow fragment of DNA polymerase I, consumes little product, requires no product purification and takes under 30 minutes.

Polymerases:

Klenow fragment

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