Klenow fragment

From E. coli (aliases:Klenow fragment, KF, Klenow fragment of E. coli DNA polymerase I, Klenow fragment of Pol I, Klenow, Pol Ik, Pol I(KF))
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This page presents all the information in Polbase for Klenow fragment.

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Structures/Sequence:

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References:

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Results:

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Klenow fragment is a family A enzyme from E. coli.

Original enzyme used in PCR (since replaced by thermostable enzymes like Taq)

Original enzyme used in chain-terminator sequencing (Sanger)

First DNA polymerase crystal structure

Originally produced by protease digestion of DNA Pol I; subsequently made from a cloned construct.

Many of the papers in the collection describe studies using exo-minus Klenow fragment.  No attempt has been made to separate these out.

Selected Properties for Klenow fragment:

3'-5' exo activity 5'-3' exo activity Frameshift Rate Substitution Rate General Error Rate
Yes (16) No (8) 6.0e-07-1.0e-02 errors/bp (3) 5.8e-06-2.8e-03 errors/bp (6) 4.0e-05-5.2e-03 errors/bp (6)
Year Authors Title Results by property
2013 Leena Maddukuri, Sarah C Shuck, Robert Lawton Eoff, Linlin Zhao, Carmelo J Rizzo, F Peter Guengerich, Lawrence J Marnett Maddukuri L2013
2013 Junpei Yamamoto, Tomoko Oyama, Tomohiro Kunishi, Chikahide Masutani, Fumio Hanaoka, Shigenori Iwai Yamamoto J2013
2013 Hongyun Wang, Nicholas Hurt, William B Dunbar Wang H2013
2013 Ping Xie Xie P2013
2012 Prakasha Gowda A S, Gowdahalli Krishnegowda, Zucai Suo, Shantu G Amin, Thomas E Spratt A S PG2012
2012 Sarah E Graham, FatimaSultana Syeda, G Andrés Cisneros Graham SE2012
2011 Oya Bermek, Nigel D F Grindley, Catherine M Joyce Bermek O2011
2011 Daniel R Garalde, Christopher A Simon, Joseph M Dahl, Hongyun Wang, Mark Akeson, Kate R Lieberman Garalde DR2011
2011 M Hogg, AE Sauer-Eriksson, Erik Johansson Hogg M2011
2011 Chen Song, Chen Zhang, Meiping Zhao Song C2011
2010 Ken Hirano, Yuichiro Yoshida, Tomomi Ishido, Yukihisa Wada, Naoji Moriya, Naoshi Yamazaki, Yoshiyuki Mizushina, Yoshinobu Baba, Mitsuru Ishikawa Hirano K2010
2010 Linda J Reha-Krantz Reha-Krantz LJ2010
2010 Guojie Zhao, Yifu Guan Zhao G2010
2009 Md Monsur Ali, Shuhei Imoto, Yingqian Li, Shigeki Sasaki, Fumi Nagatsugi Ali MM2009
2009 Gudrun Stengel, Byron W Purse, L Marcus Wilhelmsson, Milan Urban, Robert D Kuchta Stengel G2009
2009 Noriaki Minakawa, Shintaro Ogata, Mayumi Takahashi, Akira Matsuda Minakawa N2009
2009 Brett Gyarfas, Felix Olasagasti, Seico Benner, Daniel R Garalde, Kate R Lieberman, Mark Akeson Gyarfas B2009
2009 Peter Sandin, Gudrun Stengel, Thomas Ljungdahl, Karl Börjesson, Bertil Macao, L Marcus Wilhelmsson Sandin P2009 Vmax,Methods Featured,Incorporation of non-standard nucleotides,All properties
2009 Younguk Sun, Kalvin J Gregory, Val Golovlev Sun Y2009
2008 Asim Sheriff, Edward Motea, Irene Lee, Anthony J Berdis Sheriff A2008
2008 Pinky Kukreti, Kamalendra Singh, Amit Ketkar, Mukund J Modak Kukreti P2008 3-5' exo,5-3' exo,All properties
2008 Linda J Reha-Krantz Reha-Krantz LJ2008
2008 Ravindra Venkatramani, Ravi Radhakrishnan Venkatramani R2008
2008 Catherine M Joyce, Olga Potapova, Angela M DeLucia, Xuanwei Huang, Vandana Purohit Basu, Nigel D F Grindley Joyce CM2008 Cloned or native,Tagged,Tag Name,Full length or truncated,kcat,Methods Featured,All properties
2008 Janina Cramer, Gopinath Rangam, Andreas Marx, Tobias Restle Cramer J2008
2007 Michael F Bailey, Edwin J C Van der Schans, David P Millar Bailey MF2007
2007 Yasushi Shigemori, Michio Oishi Shigemori Y2007
2007 Flavia Barone, Scott D McCulloch, Peter Macpherson, Giovanni Maga, Masami Yamada, Takehiko Nohmi, Anna Minoprio, Filomena Mazzei, Thomas A Kunkel, Peter Karran, Margherita Bignami Barone F2007
2007 Adam P Silverman, Qingfei Jiang, Myron F Goodman, Eric T Kool Silverman AP2007
2007 Gudrun Stengel, JP Gill, Peter Sandin, L Marcus Wilhelmsson, Bo Albinsson, Bengt Nordén, David P Millar Stengel G2007
2006 Irene Lee, Anthony J Berdis Lee I2006
2006 Shin Mizukami, Tae Woo Kim, Sandra A Helquist, Eric T Kool Mizukami S2006
2006 Ichiro Hirao, Michiko Kimoto, Tsuneo Mitsui, Tsuyoshi Fujiwara, Rie Kawai, Akira Sato, Yoko Harada, Shigeyuki Yokoyama Hirao I2006
2006 Changbei Ma, Zhiwen Tang, Kemin Wang, Weihong Tan, Jun Li, Wei Li, Zhongjie Li, Xiaohai Yang, Huimin Li, Lingfeng Liu Ma C2006
2005 Alexander B Kotlyar, Natalia Borovok, Tatiana Molotsky, Dmitry Klinov, Benjamin Dwir, Eli Kapon Kotlyar AB2005 3-5' exo,5-3' exo,All properties
2005 Kamalendra Singh, Mukund J Modak Singh K2005 3-5' exo,5-3' exo,All properties
2005 XinJing Tang, Julia L Richards, Adam E Peritz, Ivan J Dmochowski Tang X2005
2005 Alexander B Kotlyar, Natalia Borovok, Tatiana Molotsky, Ludmila Fadeev, Michael Gozin Kotlyar AB2005 3-5' exo,5-3' exo,All properties
2005 Kristi Kincaid, Jeff W Beckman, Aleksandra Zivkovic, Randall L Halcomb, Joachim W Engels, Robert D Kuchta Kincaid K2005
2005 JP Gill, Louis J Romano Gill JP2005
2004 Kazuki Tainaka, Akimitsu Okamoto, Isao Saito Tainaka K2004
2004 Muhammed F Hashim, James N Riggins, Nathalie Schnetz-Boutaud, Markus W Voehler, Michael P Stone, Lawrence J Marnett Hashim MF2004
2004 Michael B Doughty, Karam Aboudehen, Garland Anderson, Ke Li, Bob Moore, Tina Poolson Doughty MB2004
2004 Aviva S Meyer, Maureen Blandino, Thomas E Spratt Meyer AS2004
2004 C X Wang, E Zakharova, Jinming Li, Catherine M Joyce, Jianhong Wang, William H Konigsberg Wang CX2004 kcat,All properties
2003 Aashish Srivastava, Kamalendra Singh, Mukund J Modak Srivastava A2003
2003 Lisa S Chen, Michael H Bahr, Terry L Sheppard Chen LS2003
2003 Kamalendra Singh, Mukund J Modak Singh K2003
2003 Molly Chiaramonte, Chad L Moore, Kristi Kincaid, Robert D Kuchta Chiaramonte M2003
2003 Robert M Turner, Nigel D F Grindley, Catherine M Joyce Turner RM2003
2003 Vandana Purohit, Nigel D F Grindley, Catherine M Joyce Purohit V2003
2003 Alexander Krieg, Stephan Laib, Thomas Ruckstuhl, Stefan Seeger Krieg A2003
2003 Victoria H Harris, Clifford L Smith, W Jonathan Cummins, Alan L Hamilton, David P Hornby, David M Williams Harris VH2003
2003 Olga Kornyushyna, Cynthia J Burrows Kornyushyna O2003
2002 Olga Kornyushyna, Aym M Berges, James G Muller, Cynthia J Burrows Kornyushyna O2002
2002 Elizabeth H Z Thompson, Michael F Bailey, Edwin J C Van der Schans, Catherine M Joyce, David P Millar Thompson EH2002
2002 Wai-Chung Lam, Elizabeth H Z Thompson, Olga Potapova, Xiaojun Chen Sun, Catherine M Joyce, David P Millar Lam WC2002 3-5' exo,5-3' exo,All properties
2002 Yuriy O Alekseyev, Louis J Romano Alekseyev YO2002
2002 Rajiv Gangurde, Mukund J Modak Gangurde R2002
2002 Vineeta Khare, Kristin A Eckert Khare V2002 3-5' exo,5-3' exo,All properties
2002 Olga Potapova, Nigel D F Grindley, Catherine M Joyce Potapova O2002 Overall Error Rate,All properties
2001 I Terashima, Toshiro Matsuda, T W Fang, Naomi Suzuki, J Kobayashi, K Kohda, Shinya Shibutani Terashima I2001 3-5' exo,5-3' exo,All properties
2001 Holly H Hogrefe, J Cline, A E Lovejoy, K B Nielson Hogrefe HH2001 RT,Specific Activity,Processivity,Strand Displacement,3-5' exo,5-3' exo,KM,kcat,3-5' Exo Specific Activity,All properties
2001 P Zhuang, Alexander Kolbanovskiy, Shantu Amin, Nicholas E Geacintov Zhuang P2001
2001 Manabu Yasui, S Matsui, M Ihara, Y R Laxmi, Shinya Shibutani, Toshiro Matsuda Yasui M2001
2001 Thomas E Spratt Spratt TE2001
2001 Yuriy O Alekseyev, Leonid Dzantiev, Louis J Romano Alekseyev YO2001
2001 T C Lin, C X Wang, Catherine M Joyce, William H Konigsberg Lin TC2001
2001 Emmanuelle Delagoutte, Peter H von Hippel Delagoutte E2001
2000 J C Morales, Eric T Kool Morales JC2000
2000 D S Johnston, Michael P Stone Johnston DS2000
2000 Yuriy O Alekseyev, Louis J Romano Alekseyev YO2000
2000 Steven Tuske, Kamalendra Singh, Neerja Kaushik, Mukund J Modak Tuske S2000
2000 Rajiv Gangurde, Neerja Kaushik, Kamalendra Singh, Mukund J Modak Gangurde R2000
2000 T V Rao, M T Haber, Jane M Sayer, Donald M Jerina Rao TV2000
2000 Leonid Dzantiev, Louis J Romano Dzantiev L2000
2000 Leonid Dzantiev, Louis J Romano Dzantiev L2000
2000 E F da Silva, Linda J Reha-Krantz da Silva EF2000
1999 Wai-Chung Lam, Edwin J C Van der Schans, L C Sowers, David P Millar Lam WC1999
1999 C A Brautigam, K Aschheim, Thomas A Steitz Brautigam CA1999 Other Important Residues,All properties
1999 C A Brautigam, Siyang Sun, J A Piccirilli, Thomas A Steitz Brautigam CA1999
1999 Leonid Dzantiev, Louis J Romano Dzantiev L1999
1999 Olga Rechkoblit, Shantu Amin, Nicholas E Geacintov Rechkoblit O1999
1999 Dana T Minnick, Katarzyna Bebenek, Wendy P Osheroff, Robert M Turner, Mekbib Astatke, Lingfeng Liu, Thomas A Kunkel, Catherine M Joyce Minnick DT1999
1998 Wai-Chung Lam, Edwin J C Van der Schans, Catherine M Joyce, David P Millar Lam WC1998
1998 Mekbib Astatke, K Ng, Nigel D F Grindley, Catherine M Joyce Astatke M1998
1998 W S Furey, Catherine M Joyce, M A Osborne, D Klenerman, J A Peliska, S Balasubramanian Furey WS1998
1998 T E Carver, David P Millar Carver TE1998
1998 A A Purmal, J P Bond, B A Lyons, Y W Kow, Susan S Wallace Purmal AA1998
1998 Toshinori Suzuki, Masaru Yoshida, Masami Yamada, Hiroshi Ide, M Kobayashi, K Kanaori, K Tajima, K Makino Suzuki T1998
1998 Yoshiyuki Mizushina, T Ueno, Y Goto, Y Isobe, M Sako, T Fujita, Kouji Hirota, H Hayashi, Kengo Sakaguchi Mizushina Y1998
1998 Olga I Lavrik, Heinz Peter Nasheuer, Klaus Weisshart, M S Wold, Rajendra Prasad, William A Beard, Samuel H Wilson, Alain Favre Lavrik OI1998
1998 Mekbib Astatke, Nigel D F Grindley, Catherine M Joyce Astatke M1998
1998 M J Guo, S Hildbrand, C J Leumann, L W McLaughlin, M J Waring Guo MJ1998
1998 S W Graves, Allison A Johnson, Kenneth A Johnson Graves SW1998 Processivity,Kd,Vmax,All properties
1998 James R Kiefer, C Mao, J C Braman, Lorena S Beese Kiefer JR1998 Specific Activity,Processivity,3-5' exo,KM,kcat,Polymerase Catalytic Residue Amino Acids,Residues Involved in Catalysis of 3-5' Exo,All properties
1997 E Bedford, Stanley Tabor, Charles C Richardson Bedford E1997 Specific Activity,Processivity,All properties
1997 Marc M Greenberg, T J Matray Greenberg MM1997
1997 Thomas E Spratt Spratt TE1997
1997 Holly Miller, Arthur P Grollman Miller H1997 Template lesions,All properties
1996 J S King, C F Fairley, W F Morgan King JS1996
1996 B M Moore, R K Jalluri, Michael B Doughty Moore BM1996
1996 B M Moore, Ke Li, Michael B Doughty Moore BM1996
1996 T I Kolocheva, A S Levina, G A Nevinsky Kolocheva TI1996
1996 M W Frey, S T Frey, W D Horrocks, B F Kaboord, Stephen J Benkovic Frey MW1996
1996 Dana T Minnick, Mekbib Astatke, Catherine M Joyce, Thomas A Kunkel Minnick DT1996 Cloned or native,Tagged,Full length or truncated,Frameshift Error Rate,Nucleotide Substitution Rate,KM,Kd,kcat,Molecular Weight,Overall Error Rate,All properties
1995 Mekbib Astatke, Nigel D F Grindley, Catherine M Joyce Astatke M1995
1995 Hiroyuki Kamiya, Hironori Kasai Kamiya H1995 KM,Vmax,All properties
1995 Hiroyuki Kamiya, Tadamasa Ueda, T Ohgi, Akio Matsukage, Hironori Kasai Kamiya H1995
1995 M W Frey, L C Sowers, David P Millar, Stephen J Benkovic Frey MW1995
1995 R Doisy, M S Tang Doisy R1995
1995 Catherine M Joyce, V Derbyshire Joyce CM1995
1995 V Derbyshire, J K Pinsonneault, Catherine M Joyce Derbyshire V1995
1995 Katarzyna Bebenek, Thomas A Kunkel Bebenek K1995 Frameshift Error Rate,Overall Error Rate,All properties
1995 Stephen J Benkovic, Craig E Cameron Benkovic SJ1995
1995 T J Matray, K J Haxton, Marc M Greenberg Matray TJ1995
1994 T C Lin, G Karam, William H Konigsberg Lin TC1994 KM,kcat,All properties
1994 T E Carver, R A Hochstrasser, David P Millar Carver TE1994
1994 A A Purmal, Y W Kow, Susan S Wallace Purmal AA1994
1994 J G Hacia, P B Dervan, B J Wold Hacia JG1994
1994 F J Wang, L S Ripley Wang FJ1994
1994 D Simha, D Yadav, R W Rzepka, V A Palejwala, M Zafri Humayun Simha D1994
1994 P N Yadav, J S Yadav, Edward Arnold, Mukund J Modak Yadav PN1994
1993 H P Rappaport Rappaport HP1993
1993 A T Bankier Bankier AT1993
1993 Thomas A Steitz, S Smerdon, J Jäger, Jianhong Wang, L A Kohlstaedt, J M Friedman, Lorena S Beese, P A Rice Steitz TA1993
1993 H Kong, R B Kucera, William E Jack Kong H1993 Processivity,KM,All properties
1993 W E Schy, L W Hertel, J S Kroin, Linda B Bloom, Myron F Goodman, Frank C Richardson Schy WE1993
1993 V Derbyshire, Mekbib Astatke, Catherine M Joyce Derbyshire V1993
1993 Linda B Bloom, M R Otto, J M Beechem, Myron F Goodman Bloom LB1993
1993 Kristin A Eckert, Thomas A Kunkel Eckert KA1993
1993 Lorena S Beese, J M Friedman, Thomas A Steitz Beese LS1993 Amino Acids Contacting NTP,All properties
1993 Lorena S Beese, V Derbyshire, Thomas A Steitz Beese LS1993 Amino Acids Contacting Template,Residues Involved in Catalysis of 3-5' Exo,Other Important Residues,All properties
1992 Catherine M Joyce, Xiaojun Chen Sun, Nigel D F Grindley Joyce CM1992
1992 P N Yadav, J S Yadav, Mukund J Modak Yadav PN1992
1992 M V Ljach, T I Kolocheva, V V Gorn, A S Levina, G A Nevinsky Ljach MV1992
1992 M Bakhanashvili, A Hizi Bakhanashvili M1992 RT,3-5' exo,All properties
1992 Daekyu Sun, Laurence H Hurley Sun D1992
1992 Linda J Reha-Krantz Reha-Krantz LJ1992
1991 Steven S Carroll, M Cowart, Stephen J Benkovic Carroll SS1991
1991 C R Guest, R A Hochstrasser, C G Dupuy, D J Allen, Stephen J Benkovic, David P Millar Guest CR1991
1991 V Derbyshire, Nigel D F Grindley, Catherine M Joyce Derbyshire V1991 Cloned or native,Tagged,Full length or truncated,Specific Activity,3-5' exo,KM,kcat,All properties
1991 M E Dahlberg, Stephen J Benkovic Dahlberg ME1991
1991 T K McDaniel, Y Huang, Jun Yin, S W Needleman, S J Meltzer McDaniel TK1991
1991 Lorena S Beese, Thomas A Steitz Beese LS1991 Residues Involved in Catalysis of 3-5' Exo,Other Important Residues,All properties
1991 B T Eger, Robert D Kuchta, Steven S Carroll, P A Benkovic, M E Dahlberg, Catherine M Joyce, Stephen J Benkovic Eger BT1991
1991 I A Lokhova, G A Nevinsky, T S Godovikova, E M Ivanova, A A Koshkin, D S Sergeev, E I Frolova, N K Rudenko, V V Khomov, V M Kavsan Lokhova IA1991
1991 G del Solar, M Espinosa del Solar G1991
1990 J Rush, William H Konigsberg Rush J1990
1990 I A Potapova, G A Nevinsky, V V Khomov, Olga I Lavrik Potapova IA1990
1990 G A Nevinskiĭ, I A Potapova, N B Tarusova, O V Khalabuda, V V Khomov Nevinskiĭ GA1990
1990 Vladimir N Podust, T O Korobeinicheva, G A Nevinsky, A S Levina, Olga I Lavrik Podust VN1990
1990 Katarzyna Bebenek, Catherine M Joyce, M P Fitzgerald, Thomas A Kunkel Bebenek K1990 3-5' exo,Frameshift Error Rate,Nucleotide Substitution Rate,Residues Involved in Catalysis of 3-5' Exo,All properties
1990 I A Potapova, G A Nevinsky, A G Veniaminova, V V Khomov, Olga I Lavrik Potapova IA1990
1990 A H Polesky, Thomas A Steitz, Nigel D F Grindley, Catherine M Joyce Polesky AH1990 3-5' exo,Molecular Weight,All properties
1990 M K Dosanjh, John M Essigmann, Myron F Goodman, B Singer Dosanjh MK1990
1989 S V Doronin, G A Nevinsky, T O Malygina, Vladimir N Podust, V V Khomov, Olga I Lavrik Doronin SV1989
1989 D J Allen, P L Darke, Stephen J Benkovic Allen DJ1989
1989 B Singer, F Chavez, S J Spengler, J T Kuśmierek, L Mendelman, Myron F Goodman Singer B1989
1989 C E Catalano, Stephen J Benkovic Catalano CE1989
1989 G A Nevinsky, A V Nemudraya, A S Levina, V V Khomov Nevinsky GA1989
1989 D J Allen, Stephen J Benkovic Allen DJ1989
1989 T A Rozovskaia, Sh Kh Minasian, M K Kukhanova, A A Kraevskiĭ, Z G Chidzhavadze Rozovskaia TA1989
1989 C Papanicolaou, L S Ripley Papanicolaou C1989 Overall Error Rate,All properties
1989 P Keohavong, William G Thilly Keohavong P1989 Nucleotide Substitution Rate,All properties
1989 Katarzyna Bebenek, Thomas A Kunkel Bebenek K1989
1988 R K Saiki, D H Gelfand, S Stoffel, S J Scharf, R Higuchi, G T Horn, K B Mullis, H A Erlich Saiki RK1988 Nucleotide Substitution Rate,All properties
1988 K R Tindall, Thomas A Kunkel Tindall KR1988 Full length or truncated,3-5' exo,Nucleotide Substitution Rate,Overall Error Rate,All properties
1988 P S Freemont, J M Friedman, Lorena S Beese, M R Sanderson, Thomas A Steitz Freemont PS1988 Residues Involved in Catalysis of 3-5' Exo,Other Important Residues,All properties
1988 H M Lim, J J Pène Lim HM1988
1988 P Keohavong, A G Kat, N F Cariello, William G Thilly Keohavong P1988
1988 V Derbyshire, P S Freemont, M R Sanderson, L Beese, J M Friedman, Catherine M Joyce, Thomas A Steitz Derbyshire V1988 Cloned or native,Tagged,Full length or truncated,Specific Activity,Residues Involved in Catalysis of 3-5' Exo,Other Important Residues,All properties
1987 Thomas A Steitz, L Beese, P S Freemont, J M Friedman, M R Sanderson Steitz TA1987
1987 M S Krug, S L Berger Krug MS1987
1986 P S Freemont, David L Ollis, Thomas A Steitz, Catherine M Joyce Freemont PS1986
1986 Thomas A Steitz, P S Freemont, David L Ollis, Catherine M Joyce, J M Grindley Steitz TA1986
1985 David L Ollis, C Kline, Thomas A Steitz Ollis DL1985
1985 David L Ollis, P Brick, R Hamlin, N G Xuong, Thomas A Steitz Ollis DL1985 Cloned or native,Tagged,Full length or truncated,Other Important Residues,All properties
1984 A P Gupta, P A Benkovic, Stephen J Benkovic Gupta AP1984
1984 V Bailly, W G Verly Bailly V1984
1983 Catherine M Joyce, Nigel D F Grindley Joyce CM1983 Cloned or native,All properties
1972 D Brutlag, Arthur Kornberg Brutlag D1972 Vmax,All properties
1972 P Setlow, D Brutlag, Arthur Kornberg Setlow P1972 Specific Activity,Extinction Coefficient,3-5' exo,5-3' exo,Molecular Weight,All properties
1972 P Setlow, Arthur Kornberg Setlow P1972 3-5' exo,5-3' exo,Molecular Weight,All properties
1970 H Klenow, I Henningsen Klenow H1970 Cloned or native,Tagged,Specific Activity,5-3' exo,Molecular Weight,All properties
All Polymerases | 143 Results

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