Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function.
Abstract:
Previous studies on the selection of bacteriophage T4 mutator mutants have been extended and a method to regulate the mutator activity of DNA polymerase mutator strains has been developed. The nucleotide changes of 17 bacteriophage T4 DNA polymerase mutations that confer a mutator phenotype and the nucleotide substitutions of several other T4 DNA polymerase mutations have been determined. The most striking observation is that the distribution of DNA polymerase mutator mutations is not random; almost all mutator mutations are located in the N-terminal half of the DNA polymerase. It has been shown that the T4 DNA polymerase shares several regions of homology at the protein sequence level with DNA polymerases of herpes, adeno and pox viruses. From studies of bacteriophage T4 and herpes DNA polymerase mutants, and from analyses of similar protein sequences from several organisms, we conclude that DNA polymerase synthetic activities are located in the C-terminal half of the DNA polymerase and that exonucleolytic activity is located nearer the N terminus.
Polymerases:
T4 G255S,T4 D131G,T4 G298D,T4 W213Q,T4 Q386Y,T4,T4 G82D,T4 N456TE881K,T4 Q478RA488V,T4 G694S,T4 Y317C,T4 V325A,T4 L309P,T4 L340P,T4 S345P,T4 W202S,T4 W202Q,T4 W202Y,T4 W213S,T4 W213Y,T4 Q386S,T4 Q386K,T4 Q430S,T4 W458S,T4 W482S,T4 Q592S,T4 W593S,T4 Q730S,T4 Q731S,T4 W844S,T4 Q430Y,T4 Q430K,T4 W458Q,T4 W458Y,T4 W458K,T4 W482Q,T4 W482Y,T4 W482K,T4 Q592K,T4 W593Q,T4 W593Y,T4 W593K,T4 Q730Y,T4 Q730K,T4 Q731Y,T4 Q731K,T4 W844Y,T4 D131N,T4 G304D
Topics:
Nucleotide Incorporation, Exonuclease Activity, Fidelity, Mutational Analysis, Structure and Structure/Function, Alignments, Methods
Status:
| new | topics/pols set | partial results | complete | validated |
Results:
| Polymerase | Reference | Property | Result | Context |
|---|---|---|---|---|
| T4 Q592S | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 0.01 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |
| T4 Q592K | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 0.1 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |
| T4 G82D | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 45 Mutation frequency (relative to WT) | Technique: ac forward mutational assay |
| T4 Y317C | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 410 Mutation frequency (relative to WT) | Technique: ac forward mutational assay |
| T4 L340P | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 210 Mutation frequency (relative to WT) | Technique: ac forward mutational assay |
| T4 S345P | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 25 Mutation frequency (relative to WT) | Technique: ac forward mutational assay |
| T4 G694S | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 3 Mutation frequency (relative to WT) | Technique: ac forward mutational assay |
| T4 L309P | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 716 Mutation frequency (relative to WT) | Technique: ac forward mutational assay |
| T4 V325A | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 105 Mutation frequency (relative to WT) | Technique: ac forward mutational assay |
| T4 N456TE881K | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 320 Mutation frequency (relative to WT) | Technique: ac forward mutational assay |
| T4 Q478RA488V | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 103 Mutation frequency (relative to WT) | Technique: ac forward mutational assay |
| T4 W213Y | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 0.15 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |
| T4 W213S | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 1 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |
| T4 W202Y | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 1 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |
| T4 W202Q | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 1 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |
| T4 W202S | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 0.2 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |
| T4 Q386S | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 1 errors/bp | Technique: T4 rII 199oc reversion assay |
| T4 Q386K | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 1 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |
| T4 Q386Y | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 0.2 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |
| T4 Q430Y | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 1 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |
| T4 Q430K | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 1 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |
| T4 Q430S | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 1 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |
| T4 W458S | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 0.05 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |
| T4 W458Q | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 0.1 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |
| T4 W458Y | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 0.2 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |
| T4 W458K | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 0.2 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |
| T4 W482S | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 1 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |
| T4 W482Q | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 6 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |
| T4 W482Y | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 1 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |
| T4 W482K | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 1 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |
| T4 Q730S | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 0.001 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |
| T4 Q730Y | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 0.1 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |
| T4 Q730K | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 0.1 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |
| T4 D131N | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 45 Mutation frequency (relative to WT) | Technique: ac forward mutational assay |
| T4 D131G | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 125 Mutation frequency (relative to WT) | Technique: ac forward mutational assay |
| T4 G255S | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 328 Mutation frequency (relative to WT) | Technique: ac forward mutational assay |
| T4 G298D | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 174 Mutation frequency (relative to WT) | Technique: ac forward mutational assay |
| T4 G304D | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 154 Mutation frequency (relative to WT) | Technique: ac forward mutational assay |
| T4 W593S | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 1 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |
| T4 W593Q | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 1 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |
| T4 W593Y | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 7 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |
| T4 W593K | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 1 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |
| T4 W844S | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 0.002 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |
| T4 W844Y | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 0.1 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |
| T4 Q731S | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 0.004 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |
| T4 Q731Y | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 0.01 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |
| T4 Q731K | Reha-Krantz LJ1988 | Nucleotide Substitution Rate | 0.01 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |