Isolation of bacteriophage T4 DNA polymerase mutator mutants.

Abstract:

More than 20 new bacteriophage T4 DNA polymerase mutants have been ...

Polymerases:

Topics:

Mutational Analysis, Structure and Structure/Function, Fidelity, Nucleotide Incorporation, Exonuclease Activity, Methods

One line summary:

T4 DNA mutator mutant polymerases discovered by described method are clustered at just two sites in the gene.

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
T4 D131G Isolation of bacteriophage T4 DNA polymerase mutator mutants. Nucleotide Substitution Rate 2728 Mutation frequency (relative to WT) Technique: ac forward mutational assay
T4 G82D Isolation of bacteriophage T4 DNA polymerase mutator mutants. Nucleotide Substitution Rate 300 Mutation frequency (relative to WT) Technique: ac forward mutational assay
T4 G82D Isolation of bacteriophage T4 DNA polymerase mutator mutants. Nucleotide Substitution Rate 92 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->GC (P7oc -> P7am))
T4 G82D Isolation of bacteriophage T4 DNA polymerase mutator mutants. Nucleotide Substitution Rate 100 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->TA(CG) (P7oc->rII+))
T4 G82D Isolation of bacteriophage T4 DNA polymerase mutator mutants. Nucleotide Substitution Rate 112 Mutation frequency (relative to WT) Technique: T4 rII P7am reversion assay (GC->TA(CG) (P7am->rII+))
T4 L340P Isolation of bacteriophage T4 DNA polymerase mutator mutants. Nucleotide Substitution Rate 380 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->GC (P7oc->P7am))
T4 L340P Isolation of bacteriophage T4 DNA polymerase mutator mutants. Nucleotide Substitution Rate 320 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->TA(CG) (P7oc->rII+))
T4 L340P Isolation of bacteriophage T4 DNA polymerase mutator mutants. Nucleotide Substitution Rate 1125 Mutation frequency (relative to WT) Technique: T4 rII P7am reversion assay (GC->TA(CG) (P7am->rII+))
T4 A89TD363N Isolation of bacteriophage T4 DNA polymerase mutator mutants. Nucleotide Substitution Rate 90 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->GC (P7oc->P7am))
T4 A89TD363N Isolation of bacteriophage T4 DNA polymerase mutator mutants. Nucleotide Substitution Rate 570 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->TA(CG) (P7oc->rII+))
T4 A89TD363N Isolation of bacteriophage T4 DNA polymerase mutator mutants. Nucleotide Substitution Rate 1900 Mutation frequency (relative to WT) Technique: T4 rII P7am reversion assay (GC->TA(CG) (P7am->rII+))
T4 Y317C Isolation of bacteriophage T4 DNA polymerase mutator mutants. Nucleotide Substitution Rate 800 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->GC (P7oc->P7am))
T4 Y317C Isolation of bacteriophage T4 DNA polymerase mutator mutants. Nucleotide Substitution Rate 1720 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->TA(CG) (P7oc->rII+))
T4 Y317C Isolation of bacteriophage T4 DNA polymerase mutator mutants. Nucleotide Substitution Rate 2110 Mutation frequency (relative to WT) Technique: T4 rII P7am reversion assay (GC->TA(CG) (P7am->rII+))
T4 Q478RA488V Isolation of bacteriophage T4 DNA polymerase mutator mutants. Nucleotide Substitution Rate 266 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->GC (P7oc->P7am))
T4 Q478RA488V Isolation of bacteriophage T4 DNA polymerase mutator mutants. Nucleotide Substitution Rate 200 Mutation frequency (relative to WT) Technique: T4 rII P7oc reversion assay (AT->TA(CG) (P7oc->rII+))
T4 Q478RA488V Isolation of bacteriophage T4 DNA polymerase mutator mutants. Nucleotide Substitution Rate 188 Mutation frequency (relative to WT) Technique: T4 rII P7am reversion assay (GC->TA(CG) (P7am->rII+))

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