Isolation of bacteriophage T4 DNA polymerase mutator mutants.
Journal of molecular biology (1986), Volume 189, Page 261
Abstract:
Polymerases:
Topics:
Mutational Analysis, Structure and Structure/Function, Fidelity, Nucleotide Incorporation, Exonuclease Activity, Methods
One line summary:
T4 DNA mutator mutant polymerases discovered by described method are clustered at just two sites in the gene.
Status:
new | topics/pols set | partial results | complete | validated |
Results:
Polymerase | Reference | Property | Result | Context |
---|---|---|---|---|
T4 D131G | Isolation of bacteriophage T4 DNA polymerase mutator mutants. | Nucleotide Substitution Rate | 2728 Mutation frequency (relative to WT) | Technique: ac forward mutational assay |
T4 G82D | Isolation of bacteriophage T4 DNA polymerase mutator mutants. | Nucleotide Substitution Rate | 300 Mutation frequency (relative to WT) | Technique: ac forward mutational assay |
T4 G82D | Isolation of bacteriophage T4 DNA polymerase mutator mutants. | Nucleotide Substitution Rate | 92 Mutation frequency (relative to WT) | Technique: T4 rII P7oc reversion assay (AT->GC (P7oc -> P7am)) |
T4 G82D | Isolation of bacteriophage T4 DNA polymerase mutator mutants. | Nucleotide Substitution Rate | 100 Mutation frequency (relative to WT) | Technique: T4 rII P7oc reversion assay (AT->TA(CG) (P7oc->rII+)) |
T4 G82D | Isolation of bacteriophage T4 DNA polymerase mutator mutants. | Nucleotide Substitution Rate | 112 Mutation frequency (relative to WT) | Technique: T4 rII P7am reversion assay (GC->TA(CG) (P7am->rII+)) |
T4 L340P | Isolation of bacteriophage T4 DNA polymerase mutator mutants. | Nucleotide Substitution Rate | 380 Mutation frequency (relative to WT) | Technique: T4 rII P7oc reversion assay (AT->GC (P7oc->P7am)) |
T4 L340P | Isolation of bacteriophage T4 DNA polymerase mutator mutants. | Nucleotide Substitution Rate | 320 Mutation frequency (relative to WT) | Technique: T4 rII P7oc reversion assay (AT->TA(CG) (P7oc->rII+)) |
T4 L340P | Isolation of bacteriophage T4 DNA polymerase mutator mutants. | Nucleotide Substitution Rate | 1125 Mutation frequency (relative to WT) | Technique: T4 rII P7am reversion assay (GC->TA(CG) (P7am->rII+)) |
T4 A89TD363N | Isolation of bacteriophage T4 DNA polymerase mutator mutants. | Nucleotide Substitution Rate | 90 Mutation frequency (relative to WT) | Technique: T4 rII P7oc reversion assay (AT->GC (P7oc->P7am)) |
T4 A89TD363N | Isolation of bacteriophage T4 DNA polymerase mutator mutants. | Nucleotide Substitution Rate | 570 Mutation frequency (relative to WT) | Technique: T4 rII P7oc reversion assay (AT->TA(CG) (P7oc->rII+)) |
T4 A89TD363N | Isolation of bacteriophage T4 DNA polymerase mutator mutants. | Nucleotide Substitution Rate | 1900 Mutation frequency (relative to WT) | Technique: T4 rII P7am reversion assay (GC->TA(CG) (P7am->rII+)) |
T4 Y317C | Isolation of bacteriophage T4 DNA polymerase mutator mutants. | Nucleotide Substitution Rate | 800 Mutation frequency (relative to WT) | Technique: T4 rII P7oc reversion assay (AT->GC (P7oc->P7am)) |
T4 Y317C | Isolation of bacteriophage T4 DNA polymerase mutator mutants. | Nucleotide Substitution Rate | 1720 Mutation frequency (relative to WT) | Technique: T4 rII P7oc reversion assay (AT->TA(CG) (P7oc->rII+)) |
T4 Y317C | Isolation of bacteriophage T4 DNA polymerase mutator mutants. | Nucleotide Substitution Rate | 2110 Mutation frequency (relative to WT) | Technique: T4 rII P7am reversion assay (GC->TA(CG) (P7am->rII+)) |
T4 Q478RA488V | Isolation of bacteriophage T4 DNA polymerase mutator mutants. | Nucleotide Substitution Rate | 266 Mutation frequency (relative to WT) | Technique: T4 rII P7oc reversion assay (AT->GC (P7oc->P7am)) |
T4 Q478RA488V | Isolation of bacteriophage T4 DNA polymerase mutator mutants. | Nucleotide Substitution Rate | 200 Mutation frequency (relative to WT) | Technique: T4 rII P7oc reversion assay (AT->TA(CG) (P7oc->rII+)) |
T4 Q478RA488V | Isolation of bacteriophage T4 DNA polymerase mutator mutants. | Nucleotide Substitution Rate | 188 Mutation frequency (relative to WT) | Technique: T4 rII P7am reversion assay (GC->TA(CG) (P7am->rII+)) |