Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant.
Canadian journal of genetics and cytology. Journal canadien de génétique et de cytologie (1984), Volume 26, Page 386
Abstract:
The effect of temperature on genetically well-defined mutational pathways was examined in the bacteriophage T4. The mutational site was a T4 rII ochre mutant which could revert to rII+ via a transversion or to the amber convertant via a transition. Temperature did not strongly affect any of the pathways examined in a wild-type background; however, increased temperature reduced the mutational activity of a mutator DNA polymerase mutant. Possible models to explain the role of temperature in mutagenesis are discussed as well as the significance of low temperatures for in vitro mutagenesis reactions.
Polymerases:
Topics:
Mutational Analysis, Fidelity, Nucleotide Incorporation, Exonuclease Activity
Status:
| new | topics/pols set | partial results | complete | validated |
Results:
| Polymerase | Reference | Property | Result | Context |
|---|---|---|---|---|
| T4 L340P | Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. | Nucleotide Substitution Rate | 2000 Mutation frequency (relative to WT) | Technique: T4 rII P7oc reversion assay (AT->GC pathway (P7oc -> P7am), 30°C) |
| T4 L340P | Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. | Nucleotide Substitution Rate | 333 Mutation frequency (relative to WT) | Technique: T4 rII P7oc reversion assay (AT->TA(CG) pathway (P7oc -> rII+), 30°C) |
| T4 L340P | Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. | Nucleotide Substitution Rate | 750 Mutation frequency (relative to WT) | Technique: T4 rII P7am reversion assay (GC->TA(CG) pathway (P7am -> rII+), 30°C) |
| T4 V325AL340P | Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. | Nucleotide Substitution Rate | 34 Mutation frequency (relative to WT) | Technique: T4 rII P7oc reversion assay (AT->GC pathway (P7oc -> P7am), 43°C) |
| T4 V325AL340P | Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. | Nucleotide Substitution Rate | 36 Mutation frequency (relative to WT) | Technique: T4 rII P7oc reversion assay (AT->TA(CG) pathway (P7oc -> rII+), 43°C) |
| T4 V325AL340P | Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. | Nucleotide Substitution Rate | 20 Mutation frequency (relative to WT) | Technique: T4 rII P7am reversion assay (GC->TA(CG) pathway (P7am -> rII+), 43°C) |
| T4 V325AL340P | Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. | Nucleotide Substitution Rate | 480 Mutation frequency (relative to WT) | Technique: T4 rII P7oc reversion assay (AT->GC pathway (P7oc -> P7am), 30°C) |
| T4 V325AL340P | Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. | Nucleotide Substitution Rate | 2500 Mutation frequency (relative to WT) | Technique: T4 rII P7oc reversion assay (AT->TA(CG) pathway (P7oc -> rII+), 30°C) |
| T4 V325AL340P | Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. | Nucleotide Substitution Rate | 500 Mutation frequency (relative to WT) | Technique: T4 rII P7am reversion assay (GC->TA(CG) pathway (P7am -> rII+), 30°C) |
| T4 V325AL340P | Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. | Nucleotide Substitution Rate | 214 Mutation frequency (relative to WT) | Technique: T4 rII P7oc reversion assay (AT->GC pathway (P7oc -> P7am), 37°C) |
| T4 V325AL340P | Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. | Nucleotide Substitution Rate | 333 Mutation frequency (relative to WT) | Technique: T4 rII P7oc reversion assay (AT->TA(CG) pathway (P7oc -> rII+), 37°C) |
| T4 V325AL340P | Effect of temperature on transition and transversion mutagenesis: characterization of wild type and a mutator T4 DNA polymerase mutant. | Nucleotide Substitution Rate | 36 Mutation frequency (relative to WT) | Technique: T4 rII P7am reversion assay (GC->TA(CG) pathway (P7am -> rII+), 37°C) |