Studies on the biochemical basis of mutation VI. Selection and characterization of a new bacteriophage T4 mutator DNA polymerase.

Reha-Krantz LJ, Bessman MJ
Journal of molecular biology (1981), Volume 145, Page 677
PubMed entry

Polymerases:

T4 A89TD363N,T4,T4 L340P

Topics:

Mutational Analysis, Kinetic Parameters, Structure and Structure/Function, Fidelity, Nucleotide Incorporation, Exonuclease Activity, Methods

One line summary:

T4 L340P (M19) was isolated by described procedure, which selects for mutator DNA polymerases. It has degraded specificity and makes errors by inserting the incorrect nucleotide more often than its wildtype counterpart. Also, many mutants are mapped in the appendix.

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
T4 A89TD363N Studies on the biochemical basis of mutation VI. Selection and characterization of a new bacteriophage T4 mutator DNA polymerase. Nucleotide Substitution Rate 80 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 L340P Studies on the biochemical basis of mutation VI. Selection and characterization of a new bacteriophage T4 mutator DNA polymerase. Nucleotide Substitution Rate 55 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
Log in to add missing results...

Entry validated by:

Log in to validate this paper...

Version:20261112

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.