Studies on the biochemical basis of mutation VI. Selection and characterization of a new bacteriophage T4 mutator DNA polymerase.

Polymerases:

Topics:

Mutational Analysis, Kinetic Parameters, Structure and Structure/Function, Fidelity, Nucleotide Incorporation, Exonuclease Activity, Methods

One line summary:

T4 L340P (M19) was isolated by described procedure, which selects for mutator DNA polymerases. It has degraded specificity and makes errors by inserting the incorrect nucleotide more often than its wildtype counterpart. Also, many mutants are mapped in the appendix.

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
T4 A89TD363N Reha-Krantz LJ1981 Nucleotide Substitution Rate 80 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 L340P Reha-Krantz LJ1981 Nucleotide Substitution Rate 55 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay

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