Studies on the biochemical basis of mutation VI. Selection and characterization of a new bacteriophage T4 mutator DNA polymerase.
Polymerases:
Topics:
Mutational Analysis, Kinetic Parameters, Structure and Structure/Function, Fidelity, Nucleotide Incorporation, Exonuclease Activity, Methods
One line summary:
T4 L340P (M19) was isolated by described procedure, which selects for mutator DNA polymerases. It has degraded specificity and makes errors by inserting the incorrect nucleotide more often than its wildtype counterpart. Also, many mutants are mapped in the appendix.
Status:
| new | topics/pols set | partial results | complete | validated |
Results:
| Polymerase | Reference | Property | Result | Context |
|---|---|---|---|---|
| T4 A89TD363N | Studies on the biochemical basis of mutation VI. Selection and characterization of a new bacteriophage T4 mutator DNA polymerase. | Nucleotide Substitution Rate | 80 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |
| T4 L340P | Studies on the biochemical basis of mutation VI. Selection and characterization of a new bacteriophage T4 mutator DNA polymerase. | Nucleotide Substitution Rate | 55 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |