Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase.

Polymerases:

Topics:

Mutational Analysis, Nucleotide Analogs / Template Lesions, Fidelity, Nucleotide Incorporation, Exonuclease Activity

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
T4 A89TD363N Reha-Krantz LJ1977 Nucleotide Substitution Rate 68 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (Host: B40(sup+1))
T4 A89TD363N Reha-Krantz LJ1977 Nucleotide Substitution Rate 100 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (Host: B40(sup+2))
T4 A89TD363N Reha-Krantz LJ1977 Nucleotide Substitution Rate 62 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (Host: B40(sup+3))
T4 A89TD363N Reha-Krantz LJ1977 Nucleotide Substitution Rate 254 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (Host: B40(sup+2))
T4 G694S Reha-Krantz LJ1977 Nucleotide Substitution Rate 27 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (Host: B40(sup+1))
T4 G694S Reha-Krantz LJ1977 Nucleotide Substitution Rate 16 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (Host: B40(sup+2))
T4 G694S Reha-Krantz LJ1977 Nucleotide Substitution Rate 20 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (Host: B40(sup+3))
T4 G694S Reha-Krantz LJ1977 Nucleotide Substitution Rate 17 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (Host: B40(sup+2))
T4 Q731S Reha-Krantz LJ1977 Nucleotide Substitution Rate 0.001 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 Q731Y Reha-Krantz LJ1977 Nucleotide Substitution Rate 0.015 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 A89TD363N/Q731S Reha-Krantz LJ1977 Nucleotide Substitution Rate 2.4 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 A89TD363N/Q731Y Reha-Krantz LJ1977 Nucleotide Substitution Rate 15 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 G694SQ731S Reha-Krantz LJ1977 Nucleotide Substitution Rate 0.12 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay

Entry validated by:

Log in to edit reference All References

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.