T4 DNA polymerase has a lower apparent Km for deoxynucleoside triphosphates complementary rather than noncomplementary to the template.

Polymerases:

T4

Topics:

Kinetic Parameters, Fidelity, Nucleotide Incorporation

One line summary:

Km for T4 DNA polymerase for complementary nucleotides are much lower than those obtained with noncomplementary nucleotides, with the homopolymers poly dA-poly dT as template-primer.

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
T4 Gillin FD1975 KM 1600uM Reaction: Nucleotide incorporation; Substrate: dCTP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Gillin FD1975 KM 1.9uM Reaction: Nucleotide incorporation; Substrate: dCTP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Gillin FD1975 KM 17uM Reaction: Nucleotide incorporation; Substrate: dATP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Gillin FD1975 KM 1.3uM Reaction: Nucleotide incorporation; Substrate: dATP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Gillin FD1975 KM 6uM Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Gillin FD1975 KM 1.8uM Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Gillin FD1975 KM 190uM Reaction: Nucleotide incorporation; Substrate: dGTP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Gillin FD1975 KM 1.3uM Reaction: Nucleotide incorporation; Substrate: dGTP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Gillin FD1975 Vmax 60 /minute Reaction: Nucleotide incorporation; Substrate: dGTP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Gillin FD1975 Vmax 60 /minute Reaction: Nucleotide incorporation; Substrate: dCTP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Gillin FD1975 Vmax 800 /minute Reaction: Nucleotide incorporation; Substrate: dATP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Gillin FD1975 Vmax 1120 /minute Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Gillin FD1975 Vmax 80 /minute Reaction: Nucleotide incorporation; Substrate: dGTP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Gillin FD1975 Vmax 160 /minute Reaction: Nucleotide incorporation; Substrate: dCTP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Gillin FD1975 Vmax 50 /minute Reaction: Nucleotide incorporation; Substrate: dATP; Experimental conditions: pH (pH 8.8), Temp (30°C)
T4 Gillin FD1975 Vmax 54 /minute Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: pH (pH 8.8), Temp (30°C)

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