Purification of a 9S DNA polymerase alpha species from calf thymus.

Biochemistry (1981), Volume 20, Page 5470

Abstract:

a DNA polymerase alpha species from calf thymus has been purified 12 000-fold to near homogeneity. The enzyme sediments under high salt conditions in the preparative ultracentrifuge as a homogeneous band at 9S. The specific activity is 50 000-70 000 units/mg of protein. Polypeptides of 148 000, 59 000, and 48 000 daltons are detectable. The molecular weight as estimated from gradient gel electrophoresis is about 500 000. The 9S DNA polymerase is free from terminal nucleotidyl transferase activity and does not exhibit endonuclease or exonuclease activity. It is inhibited by low concentrations of salt, aphidicolin, and N-ethylmaleimide.

Polymerases:

Topics:

Status:

new topics/pols set partial results complete validated

Results:

No results available for this paper.

Entry validated by:

Log in to edit reference All References

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.