Purification and characterization of varicella-zoster virus-induced DNA polymerase.

Abstract:

Infection of WI-38 human fibroblasts with varicella-zoster virus led ...
Infection of WI-38 human fibroblasts with varicella-zoster virus led to the stimulation of host cell DNA polymerase synthesis and induction of a new virus-specific DNA polymerase. This virus-induced DNA polymerase was partially purified and separated from host cell enzymes by DEAE-cellulose and phosphocellulose column chromatographies. This virus-induced enzyme could be distinguished from host cell enzyme by its chromatographic behavior, template specificity, and its requirement of salt for maximal activity. The enzyme could efficiently use poly(dC).oligo(dG)12-18 as well as poly(dA).oligo(dT)12-18 as template-primers. It required Mg2+ for maximal polymerization activity and was sensitive to phosphonoacetic acid, to which host alpha- and beta-DNA polymerase were relatively resistant. In addition, this induced DNA polymerase activity was enhanced by adding 60 mM (NH4)2SO4 to the reaction mixture.

Polymerases:

Topics:

Source / Purification

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
VZV Pol I Purification and characterization of varicella-zoster virus-induced DNA polymerase. Cloned or native Native organism
VZV Pol I Purification and characterization of varicella-zoster virus-induced DNA polymerase. Tagged No
VZV Pol I Purification and characterization of varicella-zoster virus-induced DNA polymerase. Full length or truncated Full length

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