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Reference: Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH.

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Polymerase	Reference	Property	Result	Context
HIV RT	Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH.	Cloned or native	Cloned in E. coli
HIV RT	Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH.	Full length or truncated	Full length
Human Pol alpha	Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH.	Full length or truncated	Full length
HIV RT	Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH.	3-5' Exonuclease (proofreading)	No
Human Pol alpha	Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH.	Cloned or native	Insect cells
Human Pol alpha	Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH.	Specific Activity	3.27E+04 units/mg
HIV RT	Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH.	Nucleotide Substitution Rate	2.63E-05 errors/bp	Technique: Reversion (pH 6.5)
HIV RT	Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH.	Nucleotide Substitution Rate	0.00027 errors/bp	Technique: Reversion (pH 8.0)
HIV RT	Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH.	Specific Activity	1 umol/min/mg
Human Pol alpha	Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH.	Nucleotide Substitution Rate	2.38E-05 errors/bp	Technique: Reversion (pH 6.1)
Human Pol alpha	Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH.	Nucleotide Substitution Rate	0.000154 errors/bp	Technique: Reversion (pH 7.8)
Human Pol alpha	Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH.	Nucleotide Substitution Rate	0.000256 errors/bp	Technique: Reversion (pH 8.6)