Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH.

Abstract:

The accuracy of DNA synthesis catalyzed by the Thermus aquaticus DNA ...

Polymerases:

Topics:

Historical Protein Properties (MW, pI, ...), Fidelity, Exonuclease Activity, Source / Purification

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
HIV RT Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH. 3-5' Exonuclease (proofreading) No
HIV RT Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH. Cloned or native Cloned in E. coli
HIV RT Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH. Nucleotide Substitution Rate 2.63E-05 errors/bp Technique: Reversion (pH 6.5)
HIV RT Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH. Nucleotide Substitution Rate 0.00027 errors/bp Technique: Reversion (pH 8.0)
HIV RT Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH. Full length or truncated Full length
HIV RT Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH. Specific Activity 1 umol/min/mg
Human Pol alpha Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH. Cloned or native Insect cells
Human Pol alpha Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH. Nucleotide Substitution Rate 2.38E-05 errors/bp Technique: Reversion (pH 6.1)
Human Pol alpha Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH. Nucleotide Substitution Rate 0.000154 errors/bp Technique: Reversion (pH 7.8)
Human Pol alpha Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH. Nucleotide Substitution Rate 0.000256 errors/bp Technique: Reversion (pH 8.6)
Human Pol alpha Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH. Full length or truncated Full length
Human Pol alpha Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH. Specific Activity 3.27E+04 units/mg

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