Partial sequence of the DNA-dependent DNA polymerase gene of fowl adenoviruses: a reference panel for a general diagnostic PCR in poultry.

Abstract:

Adenoviruses are frequent infectious agents in different poultry species. The traditional, serological typing of new isolates by virus neutralisation tests is now in transition to be replaced by PCR and sequencing. The first PCRs, recommended for the detection of adenoviruses, had been designed to target the gene of the major capsid protein, the hexon. In birds, members of three different genera of the family Adenoviridae may occur. Accordingly, three specific hexon PCRs had to be elaborated for the detection of adenoviruses in poultry. A significantly more sensitive PCR, targeting the viral DNA-dependent DNA polymerase gene, has been described recently. This method proved to be an efficient alternative for the general detection of adenoviruses irrespective of their genus affiliation. Fowl adenoviruses (FAdVs), isolated from chicken to date, comprise twelve serotypes classified into five virus species (FAdV-A to E). The polymerase gene sequence has been determined yet only from three FAdV types representing three species. In the present work, the panel of polymerase gene sequences was completed with those of the rest of FAdVs. The newly determined sequences will facilitate the identification of new FAdV isolates as an existing species or as a putative new FAdV. Once the polymerase sequence is known, more specific PCRs for the amplification of the hexon and other genes can be designed and performed according to the preliminary species classification.

Polymerases:

Topics:

Alignments, Other, Historical Protein Properties (MW, pI, ...)

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
FAV-2 Kaján GL2011 Sequence URL http://www.ncbi.nlm.nih.gov/nuccore/HM853995
FAV-3 Kaján GL2011 Sequence URL http://www.ncbi.nlm.nih.gov/nuccore/HM853996
FAV-4 Kaján GL2011 Sequence URL http://www.ncbi.nlm.nih.gov/nuccore/HM853997
FAV-5 Kaján GL2011 Sequence URL http://www.ncbi.nlm.nih.gov/nuccore/HM853998
FAV-6 Kaján GL2011 Sequence URL http://www.ncbi.nlm.nih.gov/nuccore/HM853999
FAV-7 Kaján GL2011 Sequence URL http://www.ncbi.nlm.nih.gov/nuccore/HM854000
FAV-8a Kaján GL2011 Sequence URL http://www.ncbi.nlm.nih.gov/nuccore/HM854001
FAV-8b Kaján GL2011 Sequence URL http://www.ncbi.nlm.nih.gov/nuccore/HM854002
FAV-10 Kaján GL2011 Sequence URL http://www.ncbi.nlm.nih.gov/nuccore/HM854003
FAV-11 Kaján GL2011 Sequence URL http://www.ncbi.nlm.nih.gov/nuccore/HM854004
Log in to edit reference All References

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.