Synthesis by the DNA primase of Drosophila melanogaster of a primer with a unique chain length.


The primase associated with the DNA polymerase alpha from embryos of ...
The primase associated with the DNA polymerase alpha from embryos of Drosophila melanogaster catalyzes the synthesis of ribo-oligonucleotide primers on single-stranded M13 DNA or polydeoxythymidylate templates, which can be elongated by DNA polymerase action [Conaway, R. C. & Lehman, I. R. (1982) Proc, Natl. Acad. Sci, USA 79, 2523--2527]. The primers synthesized in a coupled primase-DNA polymerase alpha reaction with an M13 DNA template are of a unique size (15 residues); those synthesized with poly(dT) range from 8 to 15 nucleotides. Primer synthesis is initiated at multiple but nonrandom sites. Like the DNA primase of Escherichia coli and the comparable activity in intact nuclei of polyoma-infected mouse cells, the DNA primase of D. melanogaster can substitute deoxynucleotides for ribonucleotides during primer synthesis.




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