Adriamycin interactions with T4 DNA polymerase. Two modes of template-mediated inhibition.

Abstract:

We examined the effect of adriamycin on kinetics of DNA synthesis catalyzed by DNA polymerase purified from bacteriophage T4-infected Escherichia coli. Two distinct modes of enzyme inhibition occur: uncompetitive and competitive at "low" and "high" drug:DNA nucleotide molar ratios, respectively. Competitive inhibition is not observed unless an unblocked amino group is present on the sugar (daunosamine) moiety. A model is proposed to relate the enzyme inhibition kinetics to intercalative and ionic binding of adriamycin to DNA.

Polymerases:

Topics:

Mutational Analysis, Enzyme Substrate Interactions

One line summary:

Adriamycin and Daunorubicin binding to DNA have varying effects on the rate of DNA replication by the T4 DNA polymerase

Status:

new topics/pols set partial results complete validated

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