KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry.

Betz K, Malyshev DA, Lavergne T, Welte W, Diederichs K, Dwyer TJ, Ordoukhanian P, Romesberg FE, Marx A
Nature chemical biology (2012), Volume 8, Page 612
PubMed entry Publisher's site

Abstract:

Many candidate unnatural DNA base pairs have been developed, but some ...
Many candidate unnatural DNA base pairs have been developed, but some of the best-replicated pairs adopt intercalated structures in free DNA that are difficult to reconcile with known mechanisms of polymerase recognition. Here we present crystal structures of KlenTaq DNA polymerase at different stages of replication for one such pair, dNaM-d5SICS, and show that efficient replication results from the polymerase itself, inducing the required natural-like structure.

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