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Reference: Kinetics of error generation in homologous B-family DNA polymerases.
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| Polymerase | Reference | Property | Result | Context |
|---|---|---|---|---|
| T4 D112AE114A | Kinetics of error generation in homologous B-family DNA polymerases. | Kd | 11uM | Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template T) |
| T4 D112AE114A | Kinetics of error generation in homologous B-family DNA polymerases. | Kd | 915uM | Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template F) |
| T4 D112AE114A | Kinetics of error generation in homologous B-family DNA polymerases. | kcat | 361 /second | Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template T) |
| T4 D112AE114A | Kinetics of error generation in homologous B-family DNA polymerases. | kcat | 3.24 /second | Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template F) |
| T4 D324A | Kinetics of error generation in homologous B-family DNA polymerases. | Kd | 18uM | Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template T) |
| T4 D324A | Kinetics of error generation in homologous B-family DNA polymerases. | Kd | 897uM | Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template F) |
| T4 D324A | Kinetics of error generation in homologous B-family DNA polymerases. | kcat | 180 /second | Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template T) |
| T4 D324A | Kinetics of error generation in homologous B-family DNA polymerases. | kcat | 2.13 /second | Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template F) |
| RB69 D114AE116A | Kinetics of error generation in homologous B-family DNA polymerases. | Kd | 47uM | Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template T) |
| RB69 D114AE116A | Kinetics of error generation in homologous B-family DNA polymerases. | Kd | 832uM | Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template F) |
| RB69 D114AE116A | Kinetics of error generation in homologous B-family DNA polymerases. | kcat | 270 /second | Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template T) |
| RB69 D114AE116A | Kinetics of error generation in homologous B-family DNA polymerases. | kcat | 0.43 /second | Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template F) |
| RB69 D327A | Kinetics of error generation in homologous B-family DNA polymerases. | Kd | 47uM | Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template T) |
| RB69 D327A | Kinetics of error generation in homologous B-family DNA polymerases. | Kd | 1210uM | Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template F) |
| RB69 D327A | Kinetics of error generation in homologous B-family DNA polymerases. | kcat | 224 /second | Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template T) |
| RB69 D327A | Kinetics of error generation in homologous B-family DNA polymerases. | kcat | 0.53 /second | Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template F) |
| T4 D219A | Kinetics of error generation in homologous B-family DNA polymerases. | Kd | 13uM | Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template T) |
| T4 D219A | Kinetics of error generation in homologous B-family DNA polymerases. | Kd | 934uM | Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template F) |
| T4 D219A | Kinetics of error generation in homologous B-family DNA polymerases. | kcat | 402 /second | Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template T) |
| T4 D219A | Kinetics of error generation in homologous B-family DNA polymerases. | kcat | 3.36 /second | Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template F) |
| T4 D219A | Kinetics of error generation in homologous B-family DNA polymerases. | kcat | 0.56 /second | Reaction: Misincorporation; Substrate: dATP; Technique: Multiple Turnover (across template C) |
| T4 D219A | Kinetics of error generation in homologous B-family DNA polymerases. | kcat | 0.18 /second | Reaction: Misincorporation; Substrate: dTTP; Technique: Multiple Turnover (across template T) |
| RB69 D222A | Kinetics of error generation in homologous B-family DNA polymerases. | Kd | 42uM | Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template T) |
| RB69 D222A | Kinetics of error generation in homologous B-family DNA polymerases. | Kd | 1767uM | Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template F) |
| RB69 D222A | Kinetics of error generation in homologous B-family DNA polymerases. | kcat | 320 /second | Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template T) |
| RB69 D222A | Kinetics of error generation in homologous B-family DNA polymerases. | kcat | 0.97 /second | Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template F) |
| RB69 D222A | Kinetics of error generation in homologous B-family DNA polymerases. | kcat | 0.43 /second | Reaction: Misincorporation; Substrate: dATP; Technique: Multiple Turnover (across template C) |
| RB69 D222A | Kinetics of error generation in homologous B-family DNA polymerases. | kcat | 0.04 /second | Reaction: Misincorporation; Substrate: dTTP; Technique: Multiple Turnover (across template T) |