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Polymerase: Dpo4

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Polymerase Reference Property Result Context
Dpo4 Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4): an archaeal DinB-like DNA polymerase with lesion-bypass properties akin to eukaryotic poleta. Molecular Weight 4.019E+04 Dalton
Dpo4 Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4): an archaeal DinB-like DNA polymerase with lesion-bypass properties akin to eukaryotic poleta. Cloned or native Cloned in E. coli
Dpo4 Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4): an archaeal DinB-like DNA polymerase with lesion-bypass properties akin to eukaryotic poleta. Template lesions Bypasses Reaction: Nucleotide incorporation; Substrate: dATP; DNA lesion: Apurinic/Apyrimidinic (AP) site
Dpo4 Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4): an archaeal DinB-like DNA polymerase with lesion-bypass properties akin to eukaryotic poleta. Template lesions Bypasses Reaction: Nucleotide incorporation; Substrate: dATP; DNA lesion: TT Cyclobutane Pyrimidine Dimer
Dpo4 Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4): an archaeal DinB-like DNA polymerase with lesion-bypass properties akin to eukaryotic poleta. Tagged No
Dpo4 Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4): an archaeal DinB-like DNA polymerase with lesion-bypass properties akin to eukaryotic poleta. Full length or truncated Full length
Dpo4 Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4): an archaeal DinB-like DNA polymerase with lesion-bypass properties akin to eukaryotic poleta. Isoelectric Point pH 9.11
Dpo4 Crystal structure of a Y-family DNA polymerase in action: a mechanism for error-prone and lesion-bypass replication. Polymerase Catalytic Residue Amino Acids D7, D105, E106
Dpo4 Crystal structure of a Y-family DNA polymerase in action: a mechanism for error-prone and lesion-bypass replication. Cloned or native Cloned in E. coli
Dpo4 Crystal structure of a Y-family DNA polymerase in action: a mechanism for error-prone and lesion-bypass replication. Full length or truncated Full length
Dpo4 Crystal structure of a Y-family DNA polymerase in action: a mechanism for error-prone and lesion-bypass replication. Amino Acids Contacting Template V32, A42, G58
Dpo4 Crystal structure of a Y-family DNA polymerase in action: a mechanism for error-prone and lesion-bypass replication. Amino Acids Contacting NTP G41, A42, A44, A57, G58
Dpo4 Increased flexibility enhances misincorporation: temperature effects on nucleotide incorporation opposite a bulky carcinogen-DNA adduct by a Y-family DNA polymerase. Template lesions Bypasses Reaction: Nucleotide incorporation; Substrate: dNTPs; DNA lesion: 8-oxo-dG
Dpo4 Molecular basis of selectivity of nucleoside triphosphate incorporation opposite O6-benzylguanine by sulfolobus solfataricus DNA polymerase Dpo4: steady-state and pre-steady-state kinetics and x-ray crystallography of correct and incorrect pairing. KM 3uM Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State (dGTP)
Dpo4 Molecular basis of selectivity of nucleoside triphosphate incorporation opposite O6-benzylguanine by sulfolobus solfataricus DNA polymerase Dpo4: steady-state and pre-steady-state kinetics and x-ray crystallography of correct and incorrect pairing. KM 1400uM Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State (O6-BzG)
Dpo4 Molecular basis of selectivity of nucleoside triphosphate incorporation opposite O6-benzylguanine by sulfolobus solfataricus DNA polymerase Dpo4: steady-state and pre-steady-state kinetics and x-ray crystallography of correct and incorrect pairing. KM 1200uM Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Steady State (O6-BzG)
Dpo4 Molecular basis of selectivity of nucleoside triphosphate incorporation opposite O6-benzylguanine by sulfolobus solfataricus DNA polymerase Dpo4: steady-state and pre-steady-state kinetics and x-ray crystallography of correct and incorrect pairing. KM 300uM Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Steady State (O6-BzG)
Dpo4 Molecular basis of selectivity of nucleoside triphosphate incorporation opposite O6-benzylguanine by sulfolobus solfataricus DNA polymerase Dpo4: steady-state and pre-steady-state kinetics and x-ray crystallography of correct and incorrect pairing. KM 600uM Reaction: Nucleotide incorporation; Substrate: dGTP; Technique: Steady State (O6-BzG)
Dpo4 Molecular basis of selectivity of nucleoside triphosphate incorporation opposite O6-benzylguanine by sulfolobus solfataricus DNA polymerase Dpo4: steady-state and pre-steady-state kinetics and x-ray crystallography of correct and incorrect pairing. kcat 0.58 /second Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State (O6-BzG)
Dpo4 Molecular basis of selectivity of nucleoside triphosphate incorporation opposite O6-benzylguanine by sulfolobus solfataricus DNA polymerase Dpo4: steady-state and pre-steady-state kinetics and x-ray crystallography of correct and incorrect pairing. kcat 0.055 /second Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State (O6-BzG)
Dpo4 Molecular basis of selectivity of nucleoside triphosphate incorporation opposite O6-benzylguanine by sulfolobus solfataricus DNA polymerase Dpo4: steady-state and pre-steady-state kinetics and x-ray crystallography of correct and incorrect pairing. kcat 0.0066 /second Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Steady State (O6-BzG)
Dpo4 Molecular basis of selectivity of nucleoside triphosphate incorporation opposite O6-benzylguanine by sulfolobus solfataricus DNA polymerase Dpo4: steady-state and pre-steady-state kinetics and x-ray crystallography of correct and incorrect pairing. kcat 0.0025 /second Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Steady State (O6-BzG)
Dpo4 Molecular basis of selectivity of nucleoside triphosphate incorporation opposite O6-benzylguanine by sulfolobus solfataricus DNA polymerase Dpo4: steady-state and pre-steady-state kinetics and x-ray crystallography of correct and incorrect pairing. kcat 0.00083 /second Reaction: Nucleotide incorporation; Substrate: dGTP; Technique: Steady State (O6-BzG)
Dpo4 Kinetic analysis of correct nucleotide insertion by a Y-family DNA polymerase reveals conformational changes both prior to and following phosphodiester bond formation as detected by tryptophan fluorescence. KM 7.7uM Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State (Template G)
Dpo4 Kinetic analysis of correct nucleotide insertion by a Y-family DNA polymerase reveals conformational changes both prior to and following phosphodiester bond formation as detected by tryptophan fluorescence. Kd 114uM Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State (template G)
Dpo4 Kinetic analysis of correct nucleotide insertion by a Y-family DNA polymerase reveals conformational changes both prior to and following phosphodiester bond formation as detected by tryptophan fluorescence. kcat 0.8 /second Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State (Template G)
Dpo4 Low fidelity DNA synthesis by a y family DNA polymerase due to misalignment in the active site. 3-5' Exonuclease (proofreading) No
Dpo4 Low fidelity DNA synthesis by a y family DNA polymerase due to misalignment in the active site. 5-3' Exonuclease No
Dpo4 Low fidelity DNA synthesis by a y family DNA polymerase due to misalignment in the active site. Frameshift Error Rate 0.0023 errors/bp Technique: M13mp2 forward mutation assay
Dpo4 Low fidelity DNA synthesis by a y family DNA polymerase due to misalignment in the active site. Nucleotide Substitution Rate 0.0065 errors/bp Technique: M13mp2 forward mutation assay
Dpo4 Pre-steady-state kinetic studies of the fidelity of Sulfolobus solfataricus P2 DNA polymerase IV. Cloned or native Cloned in E. coli
Dpo4 Pre-steady-state kinetic studies of the fidelity of Sulfolobus solfataricus P2 DNA polymerase IV. Tagged Yes
Dpo4 Pre-steady-state kinetic studies of the fidelity of Sulfolobus solfataricus P2 DNA polymerase IV. Tag Name C-terminal 6-His
Dpo4 Pre-steady-state kinetic studies of the fidelity of Sulfolobus solfataricus P2 DNA polymerase IV. Overall Error Rate 0.00015 errors/bp
Dpo4 Pre-steady-state kinetic studies of the fidelity of Sulfolobus solfataricus P2 DNA polymerase IV. Overall Error Rate 0.0032 errors/bp
Dpo4 Pre-steady-state kinetic studies of the fidelity of Sulfolobus solfataricus P2 DNA polymerase IV. Full length or truncated Full length
Dpo4 Pre-steady-state kinetic studies of the fidelity of Sulfolobus solfataricus P2 DNA polymerase IV. Vmax 3.8 /second Reaction: Nucleotide incorporation; Substrate: dNTPs

Version:20240415

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.