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Polymerase: Dpo4

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Polymerase Reference Property Result Context
Dpo4 Boudsocq F2001 Molecular Weight 4.019E+04 Dalton
Dpo4 Boudsocq F2001 Cloned or native Cloned in E. coli
Dpo4 Boudsocq F2001 Template lesions Bypasses Reaction: Nucleotide incorporation; Substrate: dATP; DNA lesion: Apurinic/Apyrimidinic (AP) site
Dpo4 Boudsocq F2001 Template lesions Bypasses Reaction: Nucleotide incorporation; Substrate: dATP; DNA lesion: TT Cyclobutane Pyrimidine Dimer
Dpo4 Boudsocq F2001 Tagged No
Dpo4 Boudsocq F2001 Full length or truncated Full length
Dpo4 Boudsocq F2001 Isoelectric Point pH 9.11
Dpo4 Ling H2001 Polymerase Catalytic Residue Amino Acids D7, D105, E106
Dpo4 Ling H2001 Cloned or native Cloned in E. coli
Dpo4 Ling H2001 Full length or truncated Full length
Dpo4 Ling H2001 Amino Acids Contacting Template V32, A42, G58
Dpo4 Ling H2001 Amino Acids Contacting NTP G41, A42, A44, A57, G58
Dpo4 Perlow-Poehnelt RA2007 Template lesions Bypasses Reaction: Nucleotide incorporation; Substrate: dNTPs; DNA lesion: 8-oxo-dG
Dpo4 Eoff RL2007 KM 3uM Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State (dGTP)
Dpo4 Eoff RL2007 KM 1400uM Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State (O6-BzG)
Dpo4 Eoff RL2007 KM 1200uM Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Steady State (O6-BzG)
Dpo4 Eoff RL2007 KM 300uM Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Steady State (O6-BzG)
Dpo4 Eoff RL2007 KM 600uM Reaction: Nucleotide incorporation; Substrate: dGTP; Technique: Steady State (O6-BzG)
Dpo4 Eoff RL2007 kcat 0.58 /second Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State (O6-BzG)
Dpo4 Eoff RL2007 kcat 0.055 /second Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State (O6-BzG)
Dpo4 Eoff RL2007 kcat 0.0066 /second Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Steady State (O6-BzG)
Dpo4 Eoff RL2007 kcat 0.0025 /second Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Steady State (O6-BzG)
Dpo4 Eoff RL2007 kcat 0.00083 /second Reaction: Nucleotide incorporation; Substrate: dGTP; Technique: Steady State (O6-BzG)
Dpo4 Beckman JW2008 KM 7.7uM Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State (Template G)
Dpo4 Beckman JW2008 Kd 114uM Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State (template G)
Dpo4 Beckman JW2008 kcat 0.8 /second Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State (Template G)
Dpo4 Kokoska RJ2002 3-5' Exonuclease (proofreading) No
Dpo4 Kokoska RJ2002 5-3' Exonuclease No
Dpo4 Kokoska RJ2002 Frameshift Error Rate 0.0023 errors/bp Technique: M13mp2 forward mutation assay
Dpo4 Kokoska RJ2002 Nucleotide Substitution Rate 0.0065 errors/bp Technique: M13mp2 forward mutation assay
Dpo4 Fiala KA2004 Cloned or native Cloned in E. coli
Dpo4 Fiala KA2004 Tagged Yes
Dpo4 Fiala KA2004 Tag Name C-terminal 6-His
Dpo4 Fiala KA2004 Overall Error Rate 0.00015 errors/bp
Dpo4 Fiala KA2004 Overall Error Rate 0.0032 errors/bp
Dpo4 Fiala KA2004 Full length or truncated Full length
Dpo4 Fiala KA2004 Vmax 3.8 /second Reaction: Nucleotide incorporation; Substrate: dNTPs

Using Polbase tables:

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Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

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It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.