Vent

From Thermococcus litoralis (aliases:Tli)
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Welcome to the Polymerase Page

This page presents all the information in Polbase for Vent.

Mutants:

At the top of the page you'll find a map of all positions of known mutants. Text links to mutants and digestion products are listed in the Mutants section.

Structures/Sequence:

If available, a representative structure is displayed at the upper right. All known structures are listed in the Structures section.

External links:

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References:

All references that refer to this Polymerase are listed with their topics and paper's title in the References table.

Results:

All the results for this polymerase can be examined by following the "Results" link at the bottom of the page.

Help icons:

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Missing mutants?
Vent is a family B enzyme from Thermococcus litoralis.

Selected Properties for Vent:

 3'-5' exo activity 5'-3' exo activity Frameshift Rate Substitution Rate General Error Rate
Yes (6) No (3) 4.5e-05-3.4e-03 errors/bp (2) 2.4e-05-5.8e-05 errors/bp (2) 2.8e-06-6.6e-05 errors/bp (3)
Year Authors Title Results by property
2012 Tomohiro Kato, Xingguo Liang, Hiroyuki Asanuma Model of elongation of short DNA sequ...
2011 M Hogg, AE Sauer-Eriksson, Erik Johansson Promiscuous DNA synthesis by human DN...
2010 Agne Tubeleviciute, Remigijus Skirgaila Compartmentalized self-replication (C...
2009 Hana Macícková-Cahová, Michal Hocek Cleavage of adenine-modified function...
2005 Jörg S Hartig, Eric T Kool Efficient isothermal expansion of hum...
2005 Jon P Anderson, Bernhard Angerer, Lawrence A Loeb Incorporation of reporter-labeled nuc... Cloned or native,Tagged,Full length or truncated,3-5' exo,5-3' exo,Application name,All properties
2004 Andrew F Gardner, Catherine M Joyce, William E Jack Comparative kinetics of nucleotide an...
2002 Andrew F Gardner, William E Jack Acyclic and dideoxy terminator prefer...
2001 Holly H Hogrefe, J Cline, A E Lovejoy, K B Nielson DNA polymerases from hyperthermophiles. Specific Activity,Processivity,Strand Displacement,3-5' exo,5-3' exo,KM,kcat,3-5' Exo Specific Activity,Overall Error Rate,All properties
2001 Enrique Viguera, D Canceill, S Dusko Ehrlich In vitro replication slippage by DNA ... Full length or truncated,Strand Displacement,3-5' exo,All properties
1999 Andrew F Gardner, William E Jack Determinants of nucleotide sugar reco...
1996 J Cline, J C Braman, Holly H Hogrefe PCR fidelity of pfu DNA polymerase an... Overall Error Rate,All properties
1996 F B Perler, Sandeep Kumar, H Kong Thermostable DNA polymerases. Full length or truncated,Processivity,Maximum Product Length,All properties
1994 Wayne M Barnes PCR amplification of up to 35-kb DNA ...
1993 H Kong, R B Kucera, William E Jack Characterization of a DNA polymerase ... Cloned or native,Full length or truncated,Processivity,3-5' exo,5-3' exo,KM,Vmax,All properties
1992 R A Hodges, F B Perler, C J Noren, William E Jack Protein splicing removes intervening ... Extension from RNA primer,All properties
1992 H W Jannasch, C O Wirsen, S J Molyneaux, T A Langworthy Comparative Physiological Studies on ...
1992 F B Perler, D G Comb, William E Jack, L S Moran, B Qiang, R B Kucera, J Benner, B E Slatko, D O Nwankwo, S K Hempstead Intervening sequences in an Archaea D... Molecular Weight,All properties
1992 P A Garrity, B J Wold Effects of different DNA polymerases ...
1991 L L Ling, P Keohavong, C Dias, William G Thilly Optimization of the polymerase chain ... Frameshift Error Rate,Overall Error Rate,All properties
1991 P Mattila, J Korpela, T Tenkanen, K Pitkänen Fidelity of DNA synthesis by the Ther... Cloned or native,3-5' exo,5-3' exo,Frameshift Error Rate,Nucleotide Substitution Rate,All properties
1991 N F Cariello, James A Swenberg, T R Skopek Fidelity of Thermococcus litoralis DN... 3-5' exo,Nucleotide Substitution Rate,Application name,All properties
Protein sequence:

Predicted Structure Model

Predicted model AlphaFold DB Not experimentally determined

This AlphaFold DB model is predicted and not an experimentally determined structure. View AlphaFold DB entry Download predicted model (.pdb)

Confidence legend (pLDDT)

AlphaFold DB colors residues by predicted confidence. Use high-confidence regions for structural context and treat low-confidence regions cautiously.

  • Very high (pLDDT > 90) Backbone and side chains are usually reliable.
  • Confident (pLDDT 70-90) Overall fold is usually reliable, but some local detail may shift.
  • Low (pLDDT 50-70) Use with caution; this region may be flexible or uncertain.
  • Very low (pLDDT < 50) Often disordered or not confidently placed.
Name 3'-5' exo activity 5'-3' exo activity Frameshift Rate Substitution Rate General Error Rate
Vent A488L

Version:20260603

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