In vitro replication slippage by DNA polymerases from thermophilic organisms.


Replication slippage of DNA polymerases is a potential source of spontaneous genetic rearrangements in prokaryotic and eukaryotic cells. Here we show that different thermostable DNA polymerases undergo replication slippage in vitro, during single-round replication of a single-stranded DNA template carrying a hairpin structure. Low-fidelity polymerases, such as Thermus aquaticus (Taq), high-fidelity polymerases, such as Pyrococcus furiosus (Pfu) and a highly thermostable polymerase from Pyrococcus abyssi (Pyra exo(-)) undergo slippage. Thermococcus litoralis DNA polymerase (Vent) is also able to slip; however, slippage can be inhibited when its strand-displacement activity is induced. Moreover, DNA polymerases that have a constitutive strand-displacement activity, such as Bacillus stearothermophilus DNA polymerase (Bst), do not slip. Polymerases that slip during single-round replication generate hairpin deletions during PCR amplification, with the exception of Vent polymerase because its strand-displacement activity is induced under these conditions. We show that these hairpin deletions occurring during PCR are due to replication slippage, and not to a previously proposed process involving polymerization across the hairpin base.



Fidelity, Source / Purification, Exonuclease Activity, Nucleotide Incorporation


new topics/pols set partial results complete validated


Polymerase Reference Property Result Context
Pfu Viguera E2001 Overall Error Rate 1.3E-06 errors/bp
Pfu Viguera E2001 Full length or truncated Full length
Vent Viguera E2001 3-5' Exonuclease (proofreading) Yes
Vent Viguera E2001 Full length or truncated Full length
Vent Viguera E2001 Strand Displacement Yes
Bst Viguera E2001 Strand Displacement Yes
Tfu Viguera E2001 3-5' Exonuclease (proofreading) Yes
Tfu Viguera E2001 Strand Displacement Yes
Pyra exo- Viguera E2001 3-5' Exonuclease (proofreading) No
Pyra exo- Viguera E2001 Strand Displacement No

Entry validated by:

Log in to edit reference All References

Using Polbase tables:


Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).


It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.