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Polymerase: Human Pol epsilon

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Polymerase	Reference	Property	Result	Context
Human Pol epsilon	Subunit protein-affinity isolation of Drosophila DNA polymerase catalytic subunit.	3-5' Exonuclease (proofreading)	Yes
Human Pol epsilon	Biochemical and functional comparison of DNA polymerases alpha, delta, and epsilon from calf thymus.	3-5' Exonuclease (proofreading)	Yes
Human Pol epsilon	Biochemical and functional comparison of DNA polymerases alpha, delta, and epsilon from calf thymus.	5-3' Exonuclease	No
Human Pol epsilon	Biochemical and functional comparison of DNA polymerases alpha, delta, and epsilon from calf thymus.	3-5' Exo Specific Activity	426 units/mg
Human Pol epsilon	Biochemical and functional comparison of DNA polymerases alpha, delta, and epsilon from calf thymus.	Full length or truncated	Full length
Human Pol epsilon	Biochemical and functional comparison of DNA polymerases alpha, delta, and epsilon from calf thymus.	RNase H	No
Human Pol epsilon	Biochemical and functional comparison of DNA polymerases alpha, delta, and epsilon from calf thymus.	Processivity	1kb
Human Pol epsilon	Biochemical and functional comparison of DNA polymerases alpha, delta, and epsilon from calf thymus.	Specific Activity	176 units/mg	Technique: Polymerase Assay (M13 DNA)
Human Pol epsilon	Characterization of a large form of DNA polymerase delta from HeLa cells that is insensitive to proliferating cell nuclear antigen.	Molecular Weight	2.15E+05 Dalton	Technique: SDS-PAGE
Human Pol epsilon	Characterization of a large form of DNA polymerase delta from HeLa cells that is insensitive to proliferating cell nuclear antigen.	3-5' Exonuclease (proofreading)	Yes
Human Pol epsilon	Characterization of a large form of DNA polymerase delta from HeLa cells that is insensitive to proliferating cell nuclear antigen.	Cloned or native	Native organism
Human Pol epsilon	Characterization of a large form of DNA polymerase delta from HeLa cells that is insensitive to proliferating cell nuclear antigen.	Tagged	No
Human Pol epsilon	Characterization of a large form of DNA polymerase delta from HeLa cells that is insensitive to proliferating cell nuclear antigen.	Full length or truncated	Full length
Human Pol epsilon	Characterization of a large form of DNA polymerase delta from HeLa cells that is insensitive to proliferating cell nuclear antigen.	Processivity	1kb
Human Pol epsilon	Characterization of a large form of DNA polymerase delta from HeLa cells that is insensitive to proliferating cell nuclear antigen.	Specific Activity	2.4E+04 units/mg
Human Pol epsilon	Characterization of a large form of DNA polymerase delta from HeLa cells that is insensitive to proliferating cell nuclear antigen.	K_M	2.5uM	Reaction: Nucleotide incorporation; Substrate: dTTP
Human Pol epsilon	Characterization of a large form of DNA polymerase delta from HeLa cells that is insensitive to proliferating cell nuclear antigen.	K_M	3uM	Reaction: Polymerase-DNA interaction; Substrate: DNA template
Human Pol epsilon	Characterization of a large form of DNA polymerase delta from HeLa cells that is insensitive to proliferating cell nuclear antigen.	K_M	4.7uM	Reaction: Nucleotide incorporation; Substrate: dNTPs
Human Pol epsilon	Thermostable DNA polymerases.	3-5' Exonuclease (proofreading)	Yes
Human Pol epsilon	Thermostable DNA polymerases.	5-3' Exonuclease	No
Human Pol epsilon	Thermostable DNA polymerases.	Full length or truncated	Full length
Human Pol epsilon	Evolution of DNA polymerases: an inactivated polymerase-exonuclease module in Pol epsilon and a chimeric origin of eukaryotic polymerases from two classes of archaeal ancestors.	3-5' Exonuclease (proofreading)	Yes
Human Pol epsilon	Evolution of DNA polymerases: an inactivated polymerase-exonuclease module in Pol epsilon and a chimeric origin of eukaryotic polymerases from two classes of archaeal ancestors.	5-3' Exonuclease	Unspecified
Human Pol epsilon	Evidence for interplay among yeast replicative DNA polymerases alpha, delta and epsilon from studies of exonuclease and polymerase active site mutations.	3-5' Exonuclease (proofreading)	Yes
Human Pol epsilon	Evidence for interplay among yeast replicative DNA polymerases alpha, delta and epsilon from studies of exonuclease and polymerase active site mutations.	5-3' Exonuclease	No
Human Pol epsilon	DNA polymerase epsilon associates with the elongating form of RNA polymerase II and nascent transcripts.	Other accessory protein(s)	RNA polymerase II
Human Pol epsilon	Further characterization of HeLa DNA polymerase epsilon.	Cloned or native	Native organism
Human Pol epsilon	Further characterization of HeLa DNA polymerase epsilon.	Tagged	No
Human Pol epsilon	Further characterization of HeLa DNA polymerase epsilon.	Full length or truncated	Full length
Human Pol epsilon	Further characterization of HeLa DNA polymerase epsilon.	Extension from RNA primer	Yes
Human Pol epsilon	Further characterization of HeLa DNA polymerase epsilon.	Percent/Fold Effect	0 % decrease	Reaction: Nucleotide incorporation