Characterization of a large form of DNA polymerase delta from HeLa cells that is insensitive to proliferating cell nuclear antigen.

Abstract:

A large form of DNA polymerase delta from HeLa cells was recently ...

Polymerases:

Topics:

Historical Protein Properties (MW, pI, ...), Kinetic Parameters, Nucleotide Incorporation, Exonuclease Activity

Note:

switched from pol delta to pol epsilon due to change in nomenclature

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Human Pol epsilon Characterization of a large form of DNA polymerase delta from HeLa cells that is insensitive to proliferating cell nuclear antigen. Molecular Weight 2.15E+05 Dalton Technique: SDS-PAGE
Human Pol epsilon Characterization of a large form of DNA polymerase delta from HeLa cells that is insensitive to proliferating cell nuclear antigen. 3-5' Exonuclease (proofreading) Yes
Human Pol epsilon Characterization of a large form of DNA polymerase delta from HeLa cells that is insensitive to proliferating cell nuclear antigen. Cloned or native Native organism
Human Pol epsilon Characterization of a large form of DNA polymerase delta from HeLa cells that is insensitive to proliferating cell nuclear antigen. Tagged No
Human Pol epsilon Characterization of a large form of DNA polymerase delta from HeLa cells that is insensitive to proliferating cell nuclear antigen. Full length or truncated Full length
Human Pol epsilon Characterization of a large form of DNA polymerase delta from HeLa cells that is insensitive to proliferating cell nuclear antigen. Processivity 1kb
Human Pol epsilon Characterization of a large form of DNA polymerase delta from HeLa cells that is insensitive to proliferating cell nuclear antigen. Specific Activity 2.4E+04 units/mg
Human Pol epsilon Characterization of a large form of DNA polymerase delta from HeLa cells that is insensitive to proliferating cell nuclear antigen. KM 2.5uM Reaction: Nucleotide incorporation; Substrate: dTTP
Human Pol epsilon Characterization of a large form of DNA polymerase delta from HeLa cells that is insensitive to proliferating cell nuclear antigen. KM 3uM Reaction: Polymerase-DNA interaction; Substrate: DNA template
Human Pol epsilon Characterization of a large form of DNA polymerase delta from HeLa cells that is insensitive to proliferating cell nuclear antigen. KM 4.7uM Reaction: Nucleotide incorporation; Substrate: dNTPs

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