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Polymerase: T4 G255S

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Polymerase Reference Property Result Context
T4 G255S Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. Nucleotide Substitution Rate 113 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 G255S Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3' --> 5' exonuclease activities. Nucleotide Substitution Rate 58 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 G255S Bacteriophage T4 DNA polymerase mutations that confer sensitivity to the PPi analog phosphonoacetic acid. Nucleotide Substitution Rate 48 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 G255S Different behaviors in vivo of mutations in the beta hairpin loop of the DNA polymerases of the closely related phages T4 and RB69. Kd 24nM Reaction: Polymerase-DNA interaction
T4 G255S Identification of a transient excision intermediate at the crossroads between DNA polymerase extension and proofreading pathways. Nucleotide Substitution Rate 58 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 G255S Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating structure to function. Nucleotide Substitution Rate 328 Mutation frequency (relative to WT) Technique: ac forward mutational assay

Version:20240415

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.