Enzymatic synthesis of deoxyribonucleic acid. I. Preparation of substrates and partial purification of an enzyme from Escherichia coli.

Abstract:

In the foregoing paper (l), a procedure was described for purifying an enzyme (“polymerase”) from Escherichia coli which catalyzes the incorporation o f deoxyribonucleotides into DNA. The purpose o f this report is to describe the requirements for the reaction, the net synthesis o f DNA, and some chemical features of the synthesized DNA. The liberation of inorganic pyrophosphate as a consequence o f the reaction and studies on the participation of inorganic pyrophosphate in reversing the polymerization will also be presented.

Polymerases:

Topics:

Historical Protein Properties (MW, pI, ...), Source / Purification, Modulators/Inhibitors

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Eco Pol I Lehman IR1958 Optimal pH pH 8.7
Eco Pol I Lehman IR1958 Percent/Fold Effect 97 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: NaCl (200mM)
Eco Pol I Lehman IR1958 Percent/Fold Effect 90 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: KF (50mM)

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