Enzymatic synthesis of deoxyribonucleic acid. I. Preparation of substrates and partial purification of an enzyme from Escherichia coli.
The Journal of biological chemistry (1958), Volume 233, Page 163
Abstract:
Polymerases:
Topics:
Historical Protein Properties (MW, pI, ...), Modulators/Inhibitors, Source / Purification
Status:
new | topics/pols set | partial results | complete | validated |
Results:
Polymerase | Reference | Property | Result | Context |
---|---|---|---|---|
Eco Pol I | Enzymatic synthesis of deoxyribonucleic acid. I. Preparation of substrates and partial purification of an enzyme from Escherichia coli. | Optimal pH | pH 8.7 | |
Eco Pol I | Enzymatic synthesis of deoxyribonucleic acid. I. Preparation of substrates and partial purification of an enzyme from Escherichia coli. | Percent/Fold Effect | 97 % decrease | Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: NaCl (200mM) |
Eco Pol I | Enzymatic synthesis of deoxyribonucleic acid. I. Preparation of substrates and partial purification of an enzyme from Escherichia coli. | Percent/Fold Effect | 90 % decrease | Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: KF (50mM) |