Enzymatic synthesis of deoxyribonucleic acid. I. Preparation of substrates and partial purification of an enzyme from Escherichia coli.

Abstract:

In the foregoing paper (l), a procedure was described for ...

Polymerases:

Topics:

Historical Protein Properties (MW, pI, ...), Modulators/Inhibitors, Source / Purification

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Eco Pol I Enzymatic synthesis of deoxyribonucleic acid. I. Preparation of substrates and partial purification of an enzyme from Escherichia coli. Optimal pH pH 8.7
Eco Pol I Enzymatic synthesis of deoxyribonucleic acid. I. Preparation of substrates and partial purification of an enzyme from Escherichia coli. Percent/Fold Effect 97 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: NaCl (200mM)
Eco Pol I Enzymatic synthesis of deoxyribonucleic acid. I. Preparation of substrates and partial purification of an enzyme from Escherichia coli. Percent/Fold Effect 90 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: KF (50mM)

Entry validated by: