Enzymatic synthesis of deoxyribonucleic acid. II. General properties of the reaction.

Bessman MJ, Lehman IR, Simms ES, Kornberg A
The Journal of biological chemistry (1958), Volume 233, Page 171
PubMed entry

Polymerases:

Eco Pol I

Topics:

Modulators/Inhibitors, Nucleotide Incorporation

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Eco Pol I Enzymatic synthesis of deoxyribonucleic acid. II. General properties of the reaction. Percent/Fold Effect 38.5 % decrease Reaction: Pyrophosphorolysis; Substrate: DNA template; Experimental conditions: CTP (.005µM), GTP (.005µM), ATP (0µM), TTP (0µM)
Eco Pol I Enzymatic synthesis of deoxyribonucleic acid. II. General properties of the reaction. Percent/Fold Effect 99.9 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: CTP (.005µM), GTP (0µM), ATP (0µM), TTP (0µM)
Eco Pol I Enzymatic synthesis of deoxyribonucleic acid. II. General properties of the reaction. Percent/Fold Effect 95 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: Mg++ (0mM)
Eco Pol I Enzymatic synthesis of deoxyribonucleic acid. II. General properties of the reaction. Percent/Fold Effect 94.5 % decrease Reaction: Pyrophosphorolysis; Substrate: DNA template; Experimental conditions: CTP (0µM), GTP (0µM), ATP (0µM), TTP (0µM)
Eco Pol I Enzymatic synthesis of deoxyribonucleic acid. II. General properties of the reaction. Percent/Fold Effect 0 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: PP (.016mM)
Eco Pol I Enzymatic synthesis of deoxyribonucleic acid. II. General properties of the reaction. Percent/Fold Effect 94 % decrease Reaction: Pyrophosphorolysis; Substrate: DNA template; Experimental conditions: NTPs (0mM)
Eco Pol I Enzymatic synthesis of deoxyribonucleic acid. II. General properties of the reaction. Percent/Fold Effect 99.5 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: CTP (.005µM), GTP (.005µM), ATP (0µM), TTP (.005µM)
Eco Pol I Enzymatic synthesis of deoxyribonucleic acid. II. General properties of the reaction. Percent/Fold Effect 67.5 % decrease Reaction: Pyrophosphorolysis; Substrate: DNA template; Experimental conditions: CTP (.005µM), GTP (0µM), ATP (0µM), TTP (0µM)
Eco Pol I Enzymatic synthesis of deoxyribonucleic acid. II. General properties of the reaction. Percent/Fold Effect 99.8 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: CTP (.005µM), GTP (.005µM), ATP (0µM), TTP (0µM)
Eco Pol I Enzymatic synthesis of deoxyribonucleic acid. II. General properties of the reaction. Percent/Fold Effect 50 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: PP (3mM)
Eco Pol I Enzymatic synthesis of deoxyribonucleic acid. II. General properties of the reaction. Percent/Fold Effect 33 % decrease Reaction: Pyrophosphorolysis; Substrate: DNA template; Experimental conditions: CTP (.005µM), GTP (.005µM), ATP (0µM), TTP (.005µM)
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Contributor: Joshua Mazzola

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