ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID, XVI. OLIGONUCLEOTIDES AS TEMPLATES AND THE MECHANISM OF THEIR REPLICATION.

Kornberg A, BERTSCH LL, JACKSON JF, KHORANA HG
Proceedings of the National Academy of Sciences of the United States of America (1964), Volume 51, Page 315
PubMed entry Full article text

Polymerases:

Eco Pol I

Topics:

Modulators/Inhibitors, Kinetic Parameters, Structure and Structure/Function, Enzyme Substrate Interactions

Note:

Paper Discusses AT-rich DNA's priming of dAT synthesis and the kinetic components therein.

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Eco Pol I ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID, XVI. OLIGONUCLEOTIDES AS TEMPLATES AND THE MECHANISM OF THEIR REPLICATION. Other Important Residues deoxyadenylate and deoxythymidylate [(AT)3 to (AT)7]
Eco Pol I ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID, XVI. OLIGONUCLEOTIDES AS TEMPLATES AND THE MECHANISM OF THEIR REPLICATION. Percent/Fold Effect 50 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: Temp (20°C)
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Contributor: Joshua Mazzola

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