Enzymatic synthesis of deoxyribonucleic acid. 28. The pyrophosphate exchange and pyrophosphorolysis reactions of deoxyribonucleic acid polymerase.

Polymerases:

Topics:

Modulators/Inhibitors, Enzyme Substrate Interactions, Kinetic Parameters

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Eco Pol I Deutscher MP1969 KM 600nM Reaction: Pyrophosphorolysis; Substrate: DNA template; Experimental conditions: Temp (37°C)
Eco Pol I Deutscher MP1969 Ki 0.7mM Reaction: Nucleotide incorporation; Inhibitor: [PP]; Substrate: dNTPs; Experimental conditions: Temp (37°C)
Eco Pol I Deutscher MP1969 Percent/Fold Effect 50 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: PP (1mM)
Eco Pol I Deutscher MP1969 Percent/Fold Effect 85 % increase Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: PP (2mM)
Eco Pol I Deutscher MP1969 Percent/Fold Effect 0 % decrease Reaction: Pyrophosphorolysis; Substrate: DNA template; Experimental conditions: pH (pH 6.5), Temp (37°C)
Eco Pol I Deutscher MP1969 Percent/Fold Effect 95 % decrease Reaction: Pyrophosphorolysis; Substrate: DNA template; Experimental conditions: pH (pH 9), Temp (37°C)

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