Enzymatic synthesis of deoxyribonucleic acid. XXXII. Replication of duplex deoxyribonucleic acid by polymerase at a single strand break.



Nick Extension, Nucleotide Incorporation, Exonuclease Activity, Historical Protein Properties (MW, pI, ...)


new topics/pols set partial results complete validated


Polymerase Reference Property Result Context
T4 Kelly RB1970 5-3' Exonuclease No
Eco Pol I Kelly RB1970 3-5' Exonuclease (proofreading) Yes
Eco Pol I Kelly RB1970 5-3' Exonuclease Yes
Eco Pol I Kelly RB1970 Optimal pH pH 7.4
Eco Pol I Kelly RB1970 Specific Activity 1.8E+04 units/mg
Eco Pol I Kelly RB1970 Nick Extension Yes, via exo activity
Eco Pol I Kelly RB1970 Strand Displacement Yes

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