Enzymatic synthesis of deoxyribonucleic acid. XXXV. A 3'-hydroxylribonucleotide binding site of Escherichia coli deoxyribonucleic acid polymerase.

Polymerases:

Topics:

Historical Protein Properties (MW, pI, ...), Enzyme Substrate Interactions, Exonuclease Activity, Kinetic Parameters

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Eco Pol I Huberman JA1970 3-5' Exonuclease (proofreading) Yes
Eco Pol I Huberman JA1970 5-3' Exonuclease Yes
Eco Pol I Huberman JA1970 Extinction Coefficient 9.26x10^4 M^-1 cm^-1 at 280nm
Eco Pol I Huberman JA1970 Kd 4000uM Reaction: Polymerase - NTP interaction; Substrate: 3’-O-methyl-fluorouracil deoxyribonucleoside 5’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 3000uM Reaction: Polymerase - NTP interaction; Substrate: Thymidylyl-(5’,3’)-thymidine 5’-triphosphate ; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 2600uM Reaction: Polymerase - NTP interaction; Substrate: Deoxycytidine 3’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 87uM Reaction: Polymerase - NTP interaction; Substrate: 5-fluorouracil deoxyribonucleoside 5'-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: pH (pH 7.4), PP (50mM)
Eco Pol I Huberman JA1970 Kd 167uM Reaction: Polymerase - NTP interaction; Substrate: Purine deoxyribonucleoside 5’-phosphate ; Technique: Equilibrium Dialysis ; Experimental conditions: pH (pH 7.4), PP (50mM)
Eco Pol I Huberman JA1970 Kd 3700uM Reaction: Polymerase - NTP interaction; Substrate: 2’,3’-dideoxythymidine 5’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 2800uM Reaction: Polymerase - NTP interaction; Substrate: Thymidine 5’-phosphonate ; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 2600uM Reaction: Polymerase - NTP interaction; Substrate: 2’-deoxyadenosine; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 3000uM Reaction: Polymerase - NTP interaction; Substrate: 3'-O-methyl AMP; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 23uM Reaction: Polymerase - NTP interaction; Substrate: 4’-deoxy-4’-thioadenine ribonucleoside 5’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 69uM Reaction: Polymerase - NTP interaction; Substrate: 6-methylmcrcaptopurinc ribonuclcosidc 5’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 144uM Reaction: Polymerase - NTP interaction; Substrate: 6-mercaptopurine ribonucleoside 5’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 1200uM Reaction: Polymerase - NTP interaction; Substrate: Deoxyadenosine 3’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 3900uM Reaction: Polymerase - NTP interaction; Substrate: Adenine-2’-deoxylyxonucleoside 5’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 3400uM Reaction: Polymerase - NTP interaction; Substrate: 2’,3’-dideoxy-2’,3’-didehydroadenosine 5’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 3900uM Reaction: Polymerase - NTP interaction; Substrate: Adenine xylonucleoside 5’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 3100uM Reaction: Polymerase - NTP interaction; Substrate: 5'-fluorouracil arabinonucleoside 5'-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 62uM Reaction: Polymerase - NTP interaction; Substrate: Aristeromycin 6'-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: pH (pH 7.4), PP (50mM)
Eco Pol I Huberman JA1970 Kd 3000uM Reaction: Polymerase - NTP interaction; Substrate: Deoxythymidine 3’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 2300uM Reaction: Polymerase - NTP interaction; Substrate: Adenine arabinonucleoside 5’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 3000uM Reaction: Polymerase - NTP interaction; Substrate: Adenosine 5’-phosphite; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 1700uM Reaction: Polymerase - NTP interaction; Substrate: 2’,3’-dideoxyadenosine 5’-phosphate ; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 2600uM Reaction: Polymerase - NTP interaction; Substrate: Thymine arabinonucleoside 5’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 3700uM Reaction: Polymerase - NTP interaction; Substrate: Uracil arabinonucleoside 5’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 2700uM Reaction: Polymerase - NTP interaction; Substrate: Adenine-3’-deoxylyxonucleoside 5’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 35uM Reaction: Polymerase - NTP interaction; Substrate: AMP; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 3000uM Reaction: Polymerase - NTP interaction; Substrate: Uracil lyxonucleoside 5’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 4200uM Reaction: Polymerase - NTP interaction; Substrate: Barbituric acid ribonucleoside 5’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 3400uM Reaction: Polymerase - NTP interaction; Substrate: Thymidine 3’,5’-diphosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 1000uM Reaction: Polymerase - NTP interaction; Substrate: Deoxy-guanosine 3’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 2300uM Reaction: Polymerase - NTP interaction; Substrate: Uracil xylonucleoside 5’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 900uM Reaction: Polymerase - NTP interaction; Substrate: Nicotinamide ribonucleoside 5’-phosphate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)
Eco Pol I Huberman JA1970 Kd 2800uM Reaction: Polymerase - NTP interaction; Substrate: guanosine 5’-methane phosphonate; Technique: Equilibrium Dialysis ; Experimental conditions: PP (50mM), pH (pH 7.4)

Entry validated by:

Log in to edit reference All References

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.