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Reference: Catalyzing "hot" reactions: enzymes from hyperthermophilic Archaea.
Polymerase | Reference | Property | Result | Context |
---|---|---|---|---|
KOD1 | Catalyzing "hot" reactions: enzymes from hyperthermophilic Archaea. | 3-5' Exonuclease (proofreading) | Yes | |
KOD1 | Catalyzing "hot" reactions: enzymes from hyperthermophilic Archaea. | Residues Involved in Catalysis of 3-5' Exo | 141Asp-Ile-Glu143 (Exo I), 210Asn-Gly-Asp-Asn-Phe-Asp215 (Exo II), 311Try-Ser-Met-Glu-Asp315 (Exo III) | |
KOD1 | Catalyzing "hot" reactions: enzymes from hyperthermophilic Archaea. | Processivity | 300bp | |
KOD1 | Catalyzing "hot" reactions: enzymes from hyperthermophilic Archaea. | Vmax | 138 /second | Reaction: Nucleotide incorporation; Substrate: dNTPs |
Pfu | Catalyzing "hot" reactions: enzymes from hyperthermophilic Archaea. | Vmax | 25 /second | Reaction: Nucleotide incorporation; Substrate: dNTPs |
PGBD Pol I | Catalyzing "hot" reactions: enzymes from hyperthermophilic Archaea. | Vmax | 23 /second | Reaction: Nucleotide incorporation; Substrate: dNTPs |
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