Welcome to the Polymerase Page

This page presents all the information in Polbase for Pfu.


At the top of the page you'll find a map of all positions of known mutants. Text links to mutants and digestion products are listed in the Mutants section.


If available, a representative structure is displayed at the upper right. All known structures are listed in the Structures section.

External links:

Polbase polymerases are linked to UniProt and Genbank where possible. The UniProt search link directs you to a pre-constructed UniProt search containing relevant terms.


All references that refer to this Polymerase are listed with their topics and paper's title in the References table.


All the results for this polymerase can be examined by following the "Results" link at the bottom of the page.

Help icons:

The show help symbol in the upper-right corner of the page links to this help text. The question mark icon is used everywhere to indicate that help is available.

Pfu is a family B enzyme from Pyrococcus furiosus.

Selected Properties for Pfu:

3'-5' exo activity 5'-3' exo activity Frameshift Rate Substitution Rate General Error Rate
Yes (4) No (1) no data 1.6e-06 errors/bp (1) 1.3e-06-3.5e-06 errors/bp (6)
Year Authors Title Results by property
2014 Fuming Sang, Yang Yang, Ying Lin, Zhizhou Zhang Sang F2014
2013 Brian J Keith, Stanislaw K Jozwiakowski, Bernard A Connolly Keith BJ2013 Overall Error Rate,All properties
2011 Connie J Hansen, Lydia Wu, Jeffrey D Fox, Bahram Arezi, Holly H Hogrefe Hansen CJ2011
2011 L-P Sun, S Wang, Z-W Zhang, Y-Y Ma, Y-Q Lai, J Weng, Q-Q Zhang Sun LP2011
2011 Xi-Peng Liu, Jian-Hua Liu Liu XP2011
2010 Morten H H Nørholm Nørholm MH2010
2009 Suk-Tae Kwon, Hyekyung Shim, Jin Choia Jeong, Jae Kima Yun, Jae-Geun Songa, Eui-Joon Kila, Il Lee Jong Kwon Suk-Tae2009 Vmax,Overall Error Rate,All properties
2009 Henry J Russell, Tomas T Richardson, Kieran Emptage, Bernard A Connolly Russell HJ2009 3-5' exo,5-3' exo,All properties
2008 Suhng Wook Kim, Dong-Uk Kim, Jin Kwang Kim, Lin-Woo Kang, Hyun-Soo Cho Kim SW2008
2008 Linda J Reha-Krantz Reha-Krantz LJ2008
2007 Hirokazu Nishida, Maiko Tanabe, Yoshizumi Ishino, Takuji Oyama, Kosuke Morikawa Nishida H2007
2007 Sukhvinder Gill, Rory O'Neill, Richard J Lewis, Bernard A Connolly Gill S2007 Template lesions,All properties
2007 Nicolas Z Rudinger, Ramon Kranaster, Andreas Marx Rudinger NZ2007
2006 Sotirios Melissis, Nikolaos E Labrou, Yannis D Clonis Melissis S2006
2006 Hirokazu Nishida, Shigeki Matsumiya, Daisuke Tsuchiya, Yoshizumi Ishino, Kosuke Morikawa Nishida H2006
2005 Jon P Anderson, Bernhard Angerer, Lawrence A Loeb Anderson JP2005 Cloned or native,Tagged,Full length or truncated,3-5' exo,5-3' exo,Application name,All properties
2005 Yair Adereth, Kristen J Champion, Tien Hsu, Vincent Dammai Adereth Y2005
2005 Unknown2005
2005 Shaohui Yang, Xincang Li, Dongfeng Ding, Jianhua Hou, Zhaoxia Jin, Xinchun Yu, Tao Bo, Weidong Li, Minggang Li Yang S2005
2004 G Shuttleworth, M J Fogg, MR Kurpiewski, L Jen-Jacobson, Bernard A Connolly Shuttleworth G2004
2004 Benjamin D Biles, Bernard A Connolly Biles BD2004
2002 Andrew F Gardner, William E Jack Gardner AF2002
2002 Tadayuki Imanaka, Haruyuki Atomi Imanaka T2002 Vmax,All properties
2001 Holly H Hogrefe, J Cline, A E Lovejoy, K B Nielson Hogrefe HH2001 Specific Activity,Processivity,Strand Displacement,3-5' exo,5-3' exo,KM,kcat,3-5' Exo Specific Activity,Overall Error Rate,All properties
2001 Enrique Viguera, D Canceill, S Dusko Ehrlich Viguera E2001 Full length or truncated,Overall Error Rate,All properties
2000 X C Li-Sucholeiki, William G Thilly Li-Sucholeiki XC2000
2000 S J Evans, M J Fogg, A Mamone, M Davis, L H Pearl, Bernard A Connolly Evans SJ2000
2000 Kayoko Komori, Yoshizumi Ishino Komori K2000
2000 L Shao, H Shinzawa, X Zhang, D B Smith, H Watanabe, H Mitsuhashi, K Saito, T Saito, H Togashi, Tomosaburo Takahashi Shao L2000
1998 S Goldman, R Kim, L W Hung, J Jancarik, S H Kim Goldman S1998
1997 L A Haff, I P Smirnov Haff LA1997
1997 Chengping Lu, H P Erickson Lu C1997
1997 P André, A Kim, Konstantin Khrapko, William G Thilly André P1997
1996 J Cline, J C Braman, Holly H Hogrefe Cline J1996 Overall Error Rate,All properties
1995 H I Yamanaka, Y Kirii, H Ohmoto Yamanaka HI1995
1994 V Picard, E Ersdal-Badju, A Lu, S C Bock Picard V1994
1994 Wayne M Barnes Barnes WM1994
1992 P Forterre Forterre P1992
1991 K S Lundberg, D D Shoemaker, M W Adams, J M Short, J A Sorge, E J Mathur Lundberg KS1991 Cloned or native,Tagged,Full length or truncated,Specific Activity,3-5' exo,Nucleotide Substitution Rate,3-5' Exo Specific Activity,All properties
1991 E J Mathur, M W Adams, W N Callen, J M Cline Mathur EJ1991
Protein sequence:
DNA sequence:
All Polymerases | 36 Results

Using Polbase tables:


Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).


It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.