Pfu
Welcome to the Polymerase Page
This page presents all the information in Polbase for Pfu.
Mutants:At the top of the page you'll find a map of all positions of known mutants. Text links to mutants and digestion products are listed in the Mutants section.
Structures/Sequence:If available, a representative structure is displayed at the upper right. All known structures are listed in the Structures section.
External links:Polbase polymerases are linked to UniProt and Genbank where possible. The UniProt search link directs you to a pre-constructed UniProt search containing relevant terms.
References:All references that refer to this Polymerase are listed with their topics and paper's title in the References table.
Results:All the results for this polymerase can be examined by following the "Results" link at the bottom of the page.
Help icons:
The symbol in the upper-right corner of the page
links to this help text. The question mark icon is used everywhere to indicate that help is available.
Selected Properties for Pfu: |
3'-5' exo activity | 5'-3' exo activity | Frameshift Rate | Substitution Rate | General Error Rate |
|---|---|---|---|---|---|
| Yes (4) | No (1) | no data | 1.6e-06 errors/bp (1) | 1.3e-06-3.5e-06 errors/bp (6) |
| Year | Authors | Title | Results by property |
|---|---|---|---|
| 2014 | Fuming Sang, Yang Yang, Ying Lin, Zhizhou Zhang | A hot start alternative for high-fide... | |
| 2013 | Brian J Keith, Stanislaw K Jozwiakowski, Bernard A Connolly | A plasmid-based lacZα gene assay for ... | Overall Error Rate,All properties |
| 2011 | Connie J Hansen, Lydia Wu, Jeffrey D Fox, Bahram Arezi, Holly H Hogrefe | Engineered split in Pfu DNA polymeras... | |
| 2011 | L-P Sun, S Wang, Z-W Zhang, Y-Y Ma, Y-Q Lai, J Weng, Q-Q Zhang | Interaction of gold nanoparticles wit... | |
| 2011 | Xi-Peng Liu, Jian-Hua Liu | Characterization of family IV UDG fro... | |
| 2010 | Morten H H Nørholm | A mutant Pfu DNA polymerase designed ... | |
| 2009 | Suk-Tae Kwon, Hyekyung Shim, Jin Choia Jeong, Jae Kima Yun, Jae-Geun Songa, Eui-Joon Kila, Il Lee Jong | Characterization and PCR optimization... | Vmax,Overall Error Rate,All properties |
| 2009 | Henry J Russell, Tomas T Richardson, Kieran Emptage, Bernard A Connolly | The 3'-5' proofreading exonuclease of... | 3-5' exo,5-3' exo,All properties |
| 2008 | Suhng Wook Kim, Dong-Uk Kim, Jin Kwang Kim, Lin-Woo Kang, Hyun-Soo Cho | Crystal structure of Pfu, the high fi... | |
| 2008 | Linda J Reha-Krantz | Recent patents of gene sequences rela... | |
| 2007 | Hirokazu Nishida, Maiko Tanabe, Yoshizumi Ishino, Takuji Oyama, Kosuke Morikawa | Crystallization and preliminary cryst... | |
| 2007 | Sukhvinder Gill, Rory O'Neill, Richard J Lewis, Bernard A Connolly | Interaction of the family-B DNA polym... | Template lesions,All properties |
| 2007 | Nicolas Z Rudinger, Ramon Kranaster, Andreas Marx | Hydrophobic amino acid and single-ato... | |
| 2006 | Sotirios Melissis, Nikolaos E Labrou, Yannis D Clonis | Nucleotide-mimetic synthetic ligands ... | |
| 2006 | Hirokazu Nishida, Shigeki Matsumiya, Daisuke Tsuchiya, Yoshizumi Ishino, Kosuke Morikawa | Stoichiometric complex formation by p... | |
| 2005 | Jon P Anderson, Bernhard Angerer, Lawrence A Loeb | Incorporation of reporter-labeled nuc... | Cloned or native,Tagged,Full length or truncated,3-5' exo,5-3' exo,Application name,All properties |
| 2005 | Yair Adereth, Kristen J Champion, Tien Hsu, Vincent Dammai | Site-directed mutagenesis using Pfu D... | |
| 2005 | Addendum: Site-directed mutagenesis u... | ||
| 2005 | Shaohui Yang, Xincang Li, Dongfeng Ding, Jianhua Hou, Zhaoxia Jin, Xinchun Yu, Tao Bo, Weidong Li, Minggang Li | A method for filling in the cohesive ... | |
| 2004 | G Shuttleworth, M J Fogg, MR Kurpiewski, L Jen-Jacobson, Bernard A Connolly | Recognition of the pro-mutagenic base... | |
| 2004 | Benjamin D Biles, Bernard A Connolly | Low-fidelity Pyrococcus furiosus DNA ... | |
| 2002 | Andrew F Gardner, William E Jack | Acyclic and dideoxy terminator prefer... | |
| 2002 | Tadayuki Imanaka, Haruyuki Atomi | Catalyzing "hot" reactions: enzymes f... | Vmax,All properties |
| 2001 | Holly H Hogrefe, J Cline, A E Lovejoy, K B Nielson | DNA polymerases from hyperthermophiles. | Specific Activity,Processivity,Strand Displacement,3-5' exo,5-3' exo,KM,kcat,3-5' Exo Specific Activity,Overall Error Rate,All properties |
| 2001 | Enrique Viguera, D Canceill, S Dusko Ehrlich | In vitro replication slippage by DNA ... | Full length or truncated,Overall Error Rate,All properties |
| 2000 | X C Li-Sucholeiki, William G Thilly | A sensitive scanning technology for l... | |
| 2000 | S J Evans, M J Fogg, A Mamone, M Davis, L H Pearl, Bernard A Connolly | Improving dideoxynucleotide-triphosph... | |
| 2000 | Kayoko Komori, Yoshizumi Ishino | Functional interdependence of DNA pol... | |
| 2000 | L Shao, H Shinzawa, X Zhang, D B Smith, H Watanabe, H Mitsuhashi, K Saito, T Saito, H Togashi, Tomosaburo Takahashi | Diversity of hepatitis G virus within... | |
| 1998 | S Goldman, R Kim, L W Hung, J Jancarik, S H Kim | Purification, crystallization and pre... | |
| 1997 | L A Haff, I P Smirnov | Single-nucleotide polymorphism identi... | |
| 1997 | Chengping Lu, H P Erickson | Expression in Escherichia coli of the... | |
| 1997 | P André, A Kim, Konstantin Khrapko, William G Thilly | Fidelity and mutational spectrum of P... | |
| 1996 | J Cline, J C Braman, Holly H Hogrefe | PCR fidelity of pfu DNA polymerase an... | Overall Error Rate,All properties |
| 1995 | H I Yamanaka, Y Kirii, H Ohmoto | An improved phage display antibody cl... | |
| 1994 | V Picard, E Ersdal-Badju, A Lu, S C Bock | A rapid and efficient one-tube PCR-ba... | |
| 1994 | Wayne M Barnes | PCR amplification of up to 35-kb DNA ... | |
| 1992 | P Forterre | The DNA polymerase from the archaebac... | |
| 1991 | K S Lundberg, D D Shoemaker, M W Adams, J M Short, J A Sorge, E J Mathur | High-fidelity amplification using a t... | Cloned or native,Tagged,Full length or truncated,Specific Activity,3-5' exo,Nucleotide Substitution Rate,3-5' Exo Specific Activity,All properties |
| 1991 | E J Mathur, M W Adams, W N Callen, J M Cline | The DNA polymerase gene from the hype... |
| Name | 3'-5' exo activity | 5'-3' exo activity | Frameshift Rate | Substitution Rate | General Error Rate |
|---|---|---|---|---|---|
| Pfu V93Q | |||||
| Pfu D215AE143A | 6.3e-06-6.7e-06 errors/bp (2) | ||||
| Pfu D215AE143A/D473G | 1.8e-05-2.4e-05 errors/bp (2) | ||||
| Pfu D215AQ484A | |||||
| Pfu D215AN492H | |||||
| Pfu D215A | |||||
| Pfu D215AD473G | |||||
| Pfu D215AK488A | |||||
| Pfu D215AN492Y | |||||
| Pfu Q472H | |||||
| Pfu A486Y | |||||
| Pfu L490W | |||||
| Pfu Y497A | |||||
| Pfu (exo-) | 5.0e-05 errors/bp (1) |
Protein information: Uniprot P61875 search for Pfu at UniProt
Sequence information: NCBI nucleotides European nucleotide archive