Over-expression of human DNA polymerase lambda in E. coli and characterization of the recombinant enzyme.

Abstract:

BACKGROUND: DNA polymerase lambda (Pol lambda) was recently identified ...

Polymerases:

Topics:

Historical Protein Properties (MW, pI, ...), Nucleotide Incorporation, Source / Purification

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Human Pol lamba Over-expression of human DNA polymerase lambda in E. coli and characterization of the recombinant enzyme. Molecular Weight 68kD Technique: Gel Filtration
Human Pol lamba Over-expression of human DNA polymerase lambda in E. coli and characterization of the recombinant enzyme. Cloned or native Cloned in E. coli
Human Pol lamba Over-expression of human DNA polymerase lambda in E. coli and characterization of the recombinant enzyme. Tagged Yes
Human Pol lamba Over-expression of human DNA polymerase lambda in E. coli and characterization of the recombinant enzyme. Tag Name His
Human Pol lamba Over-expression of human DNA polymerase lambda in E. coli and characterization of the recombinant enzyme. Full length or truncated Full length
Human Pol lamba Over-expression of human DNA polymerase lambda in E. coli and characterization of the recombinant enzyme. Processivity 1bp
Human Pol lamba Over-expression of human DNA polymerase lambda in E. coli and characterization of the recombinant enzyme. Specific Activity 3.37 units/mg Technique: Polymerase assay (poly[d(AT)]

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