TempliPhi, phi29 DNA polymerase based rolling circle amplification of templates for DNA sequencing.

Abstract:

We have developed a novel, isothermal DNA amplification strategy that ...

Polymerases:

Topics:

Kinetic Parameters, Fidelity, Nucleotide Incorporation, Exonuclease Activity, Source / Purification

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Phi29 TempliPhi, phi29 DNA polymerase based rolling circle amplification of templates for DNA sequencing. 3-5' Exonuclease (proofreading) Yes
Phi29 TempliPhi, phi29 DNA polymerase based rolling circle amplification of templates for DNA sequencing. Cloned or native Native organism
Phi29 TempliPhi, phi29 DNA polymerase based rolling circle amplification of templates for DNA sequencing. Tagged No
Phi29 TempliPhi, phi29 DNA polymerase based rolling circle amplification of templates for DNA sequencing. Overall Error Rate 3E-06 errors/bp
Phi29 TempliPhi, phi29 DNA polymerase based rolling circle amplification of templates for DNA sequencing. Full length or truncated Full length
Phi29 TempliPhi, phi29 DNA polymerase based rolling circle amplification of templates for DNA sequencing. Maximum Product Length 800kb
Phi29 TempliPhi, phi29 DNA polymerase based rolling circle amplification of templates for DNA sequencing. Processivity 70kb
Phi29 TempliPhi, phi29 DNA polymerase based rolling circle amplification of templates for DNA sequencing. Vmax 50 /second Reaction: Nucleotide incorporation; Substrate: dNTPs

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