Results for:
Reference: DNA polymerases from hyperthermophiles.
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| Polymerase | Reference | Property | Result | Context |
|---|---|---|---|---|
| Pfu | DNA polymerases from hyperthermophiles. | 3-5' Exonuclease (proofreading) | Yes | |
| Pfu | DNA polymerases from hyperthermophiles. | 5-3' Exonuclease | No | |
| Pfu | DNA polymerases from hyperthermophiles. | 3-5' Exo Specific Activity | 935 units/mg | |
| Pfu | DNA polymerases from hyperthermophiles. | Overall Error Rate | 1.3E-06 errors/bp | |
| Pfu | DNA polymerases from hyperthermophiles. | Processivity | 10bp | |
| Pfu | DNA polymerases from hyperthermophiles. | Specific Activity | 2.6 units/mg | Technique: Polymerase Assay (calf thymus DNA) |
| Pfu | DNA polymerases from hyperthermophiles. | Specific Activity | 2 units/mg | Technique: Polymerase Assay (M13 DNA) |
| Pfu | DNA polymerases from hyperthermophiles. | KM | 0.7nM | Reaction: Polymerase-DNA interaction; Substrate: DNA template |
| Pfu | DNA polymerases from hyperthermophiles. | KM | 16uM | Reaction: Nucleotide incorporation; Substrate: dNTPs |
| Pfu | DNA polymerases from hyperthermophiles. | Strand Displacement | Yes | |
| Pfu | DNA polymerases from hyperthermophiles. | kcat | 560 /minute | Reaction: Nucleotide incorporation; Substrate: dNTPs |
| Pfu (exo-) | DNA polymerases from hyperthermophiles. | 3-5' Exonuclease (proofreading) | No | |
| Pfu (exo-) | DNA polymerases from hyperthermophiles. | 5-3' Exonuclease | No | |
| Pfu (exo-) | DNA polymerases from hyperthermophiles. | Overall Error Rate | 4.7E-05 errors/bp | |
| Pfu (exo-) | DNA polymerases from hyperthermophiles. | Processivity | 11bp | |
| Pfu (exo-) | DNA polymerases from hyperthermophiles. | Specific Activity | 4.1 units/mg | Technique: Polymerase Assay (calf thymus DNA) |
| Pfu (exo-) | DNA polymerases from hyperthermophiles. | Specific Activity | 2.3 units/mg | Technique: Polymerase Assay (M13 DNA) |
| Pfu (exo-) | DNA polymerases from hyperthermophiles. | KM | 0.5nM | Reaction: Polymerase-DNA interaction; Substrate: DNA template |
| Pfu (exo-) | DNA polymerases from hyperthermophiles. | KM | 12uM | Reaction: Nucleotide incorporation; Substrate: dNTPs |
| Pfu (exo-) | DNA polymerases from hyperthermophiles. | kcat | 550 /minute | Reaction: Nucleotide incorporation; Substrate: dNTPs |
| Vent | DNA polymerases from hyperthermophiles. | 3-5' Exonuclease (proofreading) | Yes | |
| Vent | DNA polymerases from hyperthermophiles. | 3-5' Exo Specific Activity | 1644 units/mg | |
| Vent | DNA polymerases from hyperthermophiles. | 5-3' Exonuclease | No | |
| Vent | DNA polymerases from hyperthermophiles. | Overall Error Rate | 2.8E-06 errors/bp | |
| Vent | DNA polymerases from hyperthermophiles. | Processivity | 11bp | |
| Vent | DNA polymerases from hyperthermophiles. | Specific Activity | 1.8 units/mg | Technique: Polymerase Assay (calf thymus DNA) |
| Vent | DNA polymerases from hyperthermophiles. | Specific Activity | 0.7 units/mg | Technique: Polymerase Assay (M13 DNA) |
| Vent | DNA polymerases from hyperthermophiles. | KM | 57uM | Reaction: Nucleotide incorporation; Substrate: dNTPs |
| Vent | DNA polymerases from hyperthermophiles. | KM | 0.1nM | Reaction: Polymerase-DNA interaction; Substrate: DNA template |
| Vent | DNA polymerases from hyperthermophiles. | Strand Displacement | Yes | |
| Vent | DNA polymerases from hyperthermophiles. | kcat | 1000 /minute | Reaction: Nucleotide incorporation; Substrate: dNTPs |
| PGBD Pol I | DNA polymerases from hyperthermophiles. | 3-5' Exonuclease (proofreading) | Yes | |
| PGBD Pol I | DNA polymerases from hyperthermophiles. | 3-5' Exo Specific Activity | 1642 units/mg | |
| PGBD Pol I | DNA polymerases from hyperthermophiles. | 5-3' Exonuclease | No | |
| PGBD Pol I | DNA polymerases from hyperthermophiles. | Overall Error Rate | 2.7E-06 errors/bp | |
| PGBD Pol I | DNA polymerases from hyperthermophiles. | KM | 50uM | Reaction: Nucleotide incorporation; Substrate: dNTPs |
| PGBD Pol I | DNA polymerases from hyperthermophiles. | KM | 0.01nM | Reaction: Polymerase-DNA interaction; Substrate: DNA template |
| PGBD Pol I | DNA polymerases from hyperthermophiles. | Strand Displacement | Yes | |
| 9egN | DNA polymerases from hyperthermophiles. | 3-5' Exonuclease (proofreading) | Yes | |
| 9egN | DNA polymerases from hyperthermophiles. | 3-5' Exo Specific Activity | 26 units/mg | |
| 9egN | DNA polymerases from hyperthermophiles. | 5-3' Exonuclease | No | |
| 9egN | DNA polymerases from hyperthermophiles. | Overall Error Rate | 5.7E-05 errors/bp | |
| 9egN | DNA polymerases from hyperthermophiles. | Specific Activity | 0.4 units/mg | Technique: Polymerase Assay (calf thymus DNA) |
| 9egN | DNA polymerases from hyperthermophiles. | Specific Activity | 2.5 units/mg | Technique: Polymerase Assay (M13 DNA) |
| 9egN | DNA polymerases from hyperthermophiles. | KM | 0.05nM | Reaction: Polymerase-DNA interaction; Substrate: DNA template |
| 9egN | DNA polymerases from hyperthermophiles. | KM | 75uM | Reaction: Nucleotide incorporation; Substrate: dNTPs |
| 9egN | DNA polymerases from hyperthermophiles. | Strand Displacement | Yes | |
| Taq pol I | DNA polymerases from hyperthermophiles. | Reverse Transcriptase Activity | Yes | |
| Taq pol I | DNA polymerases from hyperthermophiles. | 3-5' Exonuclease (proofreading) | No | |
| Taq pol I | DNA polymerases from hyperthermophiles. | 5-3' Exonuclease | Yes | |
| Taq pol I | DNA polymerases from hyperthermophiles. | Overall Error Rate | 8E-06 errors/bp | |
| Taq pol I | DNA polymerases from hyperthermophiles. | Processivity | 10bp | |
| Taq pol I | DNA polymerases from hyperthermophiles. | Specific Activity | 2.2 units/mg | Technique: Polymerase Assay (calf thymus DNA) |
| Taq pol I | DNA polymerases from hyperthermophiles. | Specific Activity | 4.9 units/mg | Technique: Polymerase Assay (M13 DNA) |
| Taq pol I | DNA polymerases from hyperthermophiles. | KM | 24uM | Reaction: Nucleotide incorporation; Substrate: dNTPs |
| Taq pol I | DNA polymerases from hyperthermophiles. | KM | 4nM | Reaction: Polymerase-DNA interaction; Substrate: DNA template |
| Taq pol I | DNA polymerases from hyperthermophiles. | Strand Displacement | Yes | |
| Taq pol I | DNA polymerases from hyperthermophiles. | kcat | 2800 /minute | Reaction: Nucleotide incorporation; Substrate: dNTPs |
| Bst LF | DNA polymerases from hyperthermophiles. | Reverse Transcriptase Activity | Yes | |
| Bst LF | DNA polymerases from hyperthermophiles. | 3-5' Exonuclease (proofreading) | No | |
| Bst LF | DNA polymerases from hyperthermophiles. | 5-3' Exonuclease | No | |
| Bst LF | DNA polymerases from hyperthermophiles. | Processivity | 111bp | |
| Bst LF | DNA polymerases from hyperthermophiles. | Specific Activity | 15 units/mg | Technique: Polymerase Assay (calf thymus DNA) |
| Bst LF | DNA polymerases from hyperthermophiles. | Specific Activity | 49 units/mg | Technique: Polymerase Assay (M13 DNA) |
| Bst LF | DNA polymerases from hyperthermophiles. | KM | 13uM | Reaction: Nucleotide incorporation; Substrate: dNTPs |
| Bst LF | DNA polymerases from hyperthermophiles. | KM | 4.2nM | Reaction: Polymerase-DNA interaction; Substrate: DNA template |
| Bst LF | DNA polymerases from hyperthermophiles. | Strand Displacement | Yes | |
| Bst LF | DNA polymerases from hyperthermophiles. | kcat | 1.15E+04 /minute | Reaction: Nucleotide incorporation; Substrate: dNTPs |
| Klenow fragment | DNA polymerases from hyperthermophiles. | Reverse Transcriptase Activity | Yes | |
| Klenow fragment | DNA polymerases from hyperthermophiles. | 3-5' Exonuclease (proofreading) | Yes | |
| Klenow fragment | DNA polymerases from hyperthermophiles. | 3-5' Exo Specific Activity | 28 units/mg | |
| Klenow fragment | DNA polymerases from hyperthermophiles. | 5-3' Exonuclease | No | |
| Klenow fragment | DNA polymerases from hyperthermophiles. | Processivity | 7bp | |
| Klenow fragment | DNA polymerases from hyperthermophiles. | Specific Activity | 0.9 units/mg | Technique: Polymerase Assay (calf thymus DNA) |
| Klenow fragment | DNA polymerases from hyperthermophiles. | Specific Activity | 0.6 units/mg | Technique: Polymerase Assay (M13 DNA) |
| Klenow fragment | DNA polymerases from hyperthermophiles. | KM | 2.3uM | Reaction: Nucleotide incorporation; Substrate: dNTPs |
| Klenow fragment | DNA polymerases from hyperthermophiles. | KM | 1.8nM | Reaction: Polymerase-DNA interaction; Substrate: DNA template |
| Klenow fragment | DNA polymerases from hyperthermophiles. | Strand Displacement | Yes | |
| Klenow fragment | DNA polymerases from hyperthermophiles. | kcat | 310 /minute | Reaction: Nucleotide incorporation; Substrate: dNTPs |