Bacteriophage T7 Deoxyribonucleic acid replication in vitro. A protein of Escherichia coli required for bacteriophage T7 DNA polymerase activity.

Abstract:

In vivo, replication of T7 DNA does not occur after infection of Escherchia coli tsnC mutants (CHAMBERLIN, M. (1974) J. Virol. 14, 509-516). In vitro, extracts of tsnC mutant E. coli infected with T7 hage are incapable of replicating duplex T7 DNA, although extracts of wild type E. coli infected with T7 phage support replication of T7 DNA. In addition, extracts of the infected tsnC mutant are deficient in T7 DNA polymerase activity. Extracts prepared from uninfected E.coli tsnC-+ cells restore the ability of the infected tsnC extracts to replicate duplex T7 DNA, and also restore normal levels of the phage DNA polymerase activity. A 12,000-dalton heat-stable protein responsible for this complementation has been purified to near homogeneity from uninfected tsnC+ extracts and it is designated "TsnC protein."

Polymerases:

T7

Topics:

Historical Protein Properties (MW, pI, ...), Accessory Proteins/Complexes

One line summary:

This paper describes a protein designated "TsnC protein" (since identified as thioredoxin) that is required for T7 DNA polymerase activity

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
T7 Modrich P1975 Cloned or native Native organism
T7 Modrich P1975 Tagged No
T7 Modrich P1975 Full length or truncated Full length
T7 Modrich P1975 Specific Activity 4100 units/mg
T7 Modrich P1975 Other accessory protein(s) TsnC protein (thioredoxin)

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