Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7.

Abstract:

Bacteriophage T7 gene 5 protein has been purified to apparent ...

Polymerases:

Topics:

Historical Protein Properties (MW, pI, ...), Kinetic Parameters, Nucleotide Incorporation, Source / Purification

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
T7 (no thioreoxin) Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7. Molecular Weight 8E+04 Dalton Technique: SDS-PAGE
T7 (no thioreoxin) Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7. Cloned or native Cloned in E. coli
T7 (no thioreoxin) Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7. Full length or truncated Full length
T7 (no thioreoxin) Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7. Processivity 1bp
T7 (no thioreoxin) Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7. Specific Activity 110 units/mg Technique: Polymerase Assay (calf thymus DNA)
T7 (no thioreoxin) Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7. Specific Activity 14 units/mg Technique: Polymerase Assay (M13 DNA)
T7 Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7. Cloned or native Cloned in E. coli
T7 Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7. Tagged No
T7 Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7. Full length or truncated Full length
T7 Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7. Processivity 10kb
T7 Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7. Specific Activity 8300 units/mg Technique: Polymerase Assay (calf thymus DNA)
T7 Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7. Specific Activity 5.3E+04 units/mg Technique: Polymerase Assay (M13 DNA)
T7 Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7. Vmax 100 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Eco Pol I Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7. Full length or truncated Full length
Eco Pol I Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7. Specific Activity 9800 units/mg Technique: Polymerase Assay (calf thymus DNA)
Eco Pol I Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7. Specific Activity 2.8E+04 units/mg Technique: Polymerase Assay (M13 DNA)

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