An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme.
Abstract:
Polymerases:
Topics:
Kinetic Parameters, Fidelity, Nucleotide Incorporation, Methods
Status:
new | topics/pols set | partial results | complete | validated |
Results:
Polymerase | Reference | Property | Result | Context |
---|---|---|---|---|
T4 | An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme. | KM | 17uM | Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: Temp (30°C) |
T4 | An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme. | KM | 17uM | Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: Temp (43°C) |
T4 | An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme. | KM | 47uM | Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: Temp (30°C) |
T4 | An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme. | Vmax | 0.33 /minute | Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: Temp (30°C) |
T4 | An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme. | Vmax | 0.66 /minute | Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: Temp (43°C) |
T4 | An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme. | Vmax | 0.15 /minute | Reaction: 3-5' Exonuclease; Substrate: DNA template |
T4 A737V | An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme. | KM | 37uM | Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: Temp (30°C) |
T4 A737V | An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme. | KM | 138uM | Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: Temp (43°C) |
T4 A737V | An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme. | KM | 47uM | Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: Temp (30°C) |
T4 A737V | An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme. | Vmax | 0.52 /minute | Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: Temp (30°C) |
T4 A737V | An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme. | Vmax | 1.72 /minute | Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: Temp (43°C) |
T4 A737V | An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme. | Vmax | 0.86 /minute | Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: Temp (30°C) |