An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme.

Abstract:

The DNA polymerase induced by an antimutator T4 phage has been ...

Polymerases:

Topics:

Kinetic Parameters, Fidelity, Nucleotide Incorporation, Methods

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
T4 An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme. KM 17uM Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: Temp (30°C)
T4 An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme. KM 17uM Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: Temp (43°C)
T4 An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme. KM 47uM Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: Temp (30°C)
T4 An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme. Vmax 0.33 /minute Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: Temp (30°C)
T4 An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme. Vmax 0.66 /minute Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: Temp (43°C)
T4 An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme. Vmax 0.15 /minute Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 A737V An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme. KM 37uM Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: Temp (30°C)
T4 A737V An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme. KM 138uM Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: Temp (43°C)
T4 A737V An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme. KM 47uM Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: Temp (30°C)
T4 A737V An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme. Vmax 0.52 /minute Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: Temp (30°C)
T4 A737V An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme. Vmax 1.72 /minute Reaction: Nucleotide incorporation; Substrate: dTTP; Experimental conditions: Temp (43°C)
T4 A737V An antimutator deoxyribonucleic acid polymerase. I. Purification and properties of the enzyme. Vmax 0.86 /minute Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: Temp (30°C)

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