Gel kinetic analysis of DNA polymerase fidelity in the presence of proofreading using bacteriophage T4 DNA polymerase.
The Journal of biological chemistry (1995), Volume 270, Page 4759
Abstract:
Polymerases:
Topics:
Kinetic Parameters, Fidelity, Exonuclease Activity
One line summary:
High concentrations of a dNTP would suppress mispairs of that type
Status:
new | topics/pols set | partial results | complete | validated |
Results:
Polymerase | Reference | Property | Result | Context |
---|---|---|---|---|
T4 | Gel kinetic analysis of DNA polymerase fidelity in the presence of proofreading using bacteriophage T4 DNA polymerase. | Nucleotide Substitution Rate | 1E-06 errors/bp | Technique: Gel kinetic assay (dCMP-A mismatch) |
T4 | Gel kinetic analysis of DNA polymerase fidelity in the presence of proofreading using bacteriophage T4 DNA polymerase. | Nucleotide Substitution Rate | 1E-05 errors/bp | Technique: Gel kinetic assay (dGMP-A mismatch) |
T4 | Gel kinetic analysis of DNA polymerase fidelity in the presence of proofreading using bacteriophage T4 DNA polymerase. | Nucleotide Substitution Rate | 1E-08 errors/bp | Technique: Gel kinetic assay (dAMP-A mismatch) |
T4 | Gel kinetic analysis of DNA polymerase fidelity in the presence of proofreading using bacteriophage T4 DNA polymerase. | Nucleotide Substitution Rate | 0.0002 errors/bp | Technique: Gel kinetic assay (dTMP-T mismatch) |