Enzymatic synthesis of deoxyribonucleic acid. XXV. Purification and properties of deoxyribonucleic acid polymerase induced by infection with phage T4.

Polymerases:

T4

Topics:

Historical Protein Properties (MW, pI, ...), Modulators/Inhibitors, Nucleotide Incorporation, Exonuclease Activity, Source / Purification

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
T4 Goulian M1968 Molecular Weight 1.12E+05 Dalton
T4 Goulian M1968 3-5' Exonuclease (proofreading) Yes
T4 Goulian M1968 5-3' Exonuclease No
T4 Goulian M1968 Full length or truncated Full length
T4 Goulian M1968 Optimal pH pH 8.5
T4 Goulian M1968 Specific Activity 3.3E+04 units/mg Technique: Polymerase Assay (calf thymus DNA)
T4 Goulian M1968 Strand Displacement No
T4 Goulian M1968 Percent/Fold Effect 0 % decrease Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: Mg++ (6mM)
T4 Goulian M1968 Percent/Fold Effect 75 % decrease Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: Mg++ (.1mM)
T4 Goulian M1968 Percent/Fold Effect 0 % decrease Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: NTPs (0mM)
T4 Goulian M1968 Percent/Fold Effect 49 % decrease Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: GTP (0.2mM), ATP (0mM), CTP (0mM), TTP (0mM)
T4 Goulian M1968 Percent/Fold Effect 81 % decrease Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: GTP (0.2mM), ATP (0.2mM), CTP (0mM)
T4 Goulian M1968 Percent/Fold Effect 96 % decrease Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: GTP (0.2mM), ATP (0.2mM), CTP (0.2mM), TTP (0mM)
T4 Goulian M1968 Gap Filling Yes

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